Pknox1^{Gt(OST71835)Lex}
Gene trapped Allele Detail

Nomenclature

• Symbol: Pknox1^{Gt(OST71835)Lex}
• Name: Pbx/knotted 1 homeobox; gene trap OST71835, Lexicon Genetics
• MGI ID: MGI:3530910
• Synonyms: Pknox1^{Gt(neo)628Lex}, Prep1ⁱ
• Gene: Pknox1 Location: Chr17:31564749-31607684 bp, + strand Genetic Position: Chr17, 16.78 cM, cytoband B-C

Mutation origin

• Mutant Cell Line: OST71835
• Germline Transmission: Earliest citation of germline transmission: J:103485
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector
• Mutation details: The clone used for gene trapping was isolated from a library of embryonic stem cells randomly targeted with the retroviral vector VICTR45 using an enhancer trap strategy. Retroviral insertion occurred at nucleotide 849 of intron 1 located within the 5' untranslated region (2.8 kb from the ATG). RT-PCR analysis revealed that transcript was absent in most homozygous mice. However, in some homozygotes a low level of protein (about 5-10% of wild-type) is detected. (J:103485, J:103769, J:111418)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST71835; (Lexicon Genetics)	CG515612 ; (MGI Seq Detail)	3' RACE	Chr17:31583696-31585390 bp (+)
  * GRCm38; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;
  Blue line marks approximate position of gene trap insertion;

  227 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

10 anatomical structures

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Pknox1 Mutation: 77 strains or lines available

Notes

See also, data as provided by Lexicon Genetics, Inc.

References

• Original: J:103485 Lexicon Genetics Inc, NIH initiative supporting placement of Lexicon Genetics, Inc. mice into public repositories. MGI Direct Data Submission. 2005
• All: 18 reference(s)