Pank3^Gt(XE134)Byg
Gene trapped Allele Detail

Summary

• Symbol: Pank3^Gt(XE134)Byg
• Name: pantothenate kinase 3; gene trap XE134, BayGenomics
• MGI ID: MGI:3521927
• Synonyms: Pank3^{Gt(pGT1Lxf)2Ucd}
• Gene: Pank3 Location: Chr11:35660322-35682112 bp, + strand Genetic Position: Chr11, 21.96 cM
• Alliance: Pank3^Gt(XE134)Byg page

Mutation origin

• Mutant Cell Line: XE134
• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The pGT1Lxf gene trap vector contains the Betageo gene, a fusion of the bacterial beta-galactosidase (lacZ) and neomycin phosphotransferase 2 (neo^r) coding sequences, downstream of a splice acceptor/exon of the engrailed 2 (En2) gene. In this instance the vector inserted correctly into an intron of the mouse gene, so that splicing of the primary transcript of the trapped gene joins its previous exon to the En2 exon. Expression from the endogenous promoter is expected to result in expression of beta-galactosidase in cells where the endogenous gene is normally expressed. (J:94303)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XE134	CC178311 ; (MGI Seq Detail)	5' RACE	Chr11:35660682-35660773 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  9 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pank3 Mutation: 21 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:94303 BayGenomics, Information obtained from the Bay Area Functional Genomics Consortium (BayGenomics). Unpublished. 2004
• All: 3 reference(s)