About   Help   FAQ
Targeted Allele Detail
Symbol: Mmp13tm1Smk
Name: matrix metallopeptidase 13; targeted mutation 1, Stephen M Krane
MGI ID: MGI:3521852
Synonyms: Mmp-13-, Mmp13-
Gene: Mmp13  Location: Chr9:7272514-7283331 bp, + strand  Genetic Position: Chr9, 2.46 cM, cytoband A1-A2
Long bone analysis from wild type and Mmp13tm1Smk/Mmp13tm1Smk mice

Show the 2 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:94723
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
Mutation detailsA targeting vector was designed to insert a PGK-neo and a stop codon in order to splice out exon 5, which encodes the zinc-binding residues in the catalytic domain. Northern blot of mutant calvariae demonstrated a lack of transcript when an exon 5 probe was used and a barely detectable amount of transcript when using a 5' probe. Western blot showed no protein in cultures of mutant calvariae. (J:94723)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mmp13 Mutation:  17 strains or lines available
Original:  J:94723 Inada M, et al., Critical roles for collagenase-3 (Mmp13) in development of growth plate cartilage and in endochondral ossification. Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17192-7
All:  14 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.11
The Jackson Laboratory