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Transgene Detail
Symbol: Tg(GFAP-APOE_i2)14Hol
Name: transgene insertion 14, David M Holtzman
MGI ID: MGI:3057185
Synonyms: GFAP-apoE2 line14, Tg(GFAP-APOE*2)14Hol, Tg(GFAP-APOE2)14Hol
Transgene: Tg(GFAP-APOE_i2)14Hol  Location: unknown  
Strain of Origin:  C57BL/6 and CBA
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(GFAP-APOE_i2)14Hol expresses 1 gene
Mutation detailsThe transgene contains sequence encoding human apolipoprotein E2 isoform under the control of the human glial fibrillary acidic protein (GFAP) promoter. (J:93487)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Transgenic mice do not express endogenous mouse apolipoprotein E (APOE). The expression pattern of the transgenic human apolipoprotein E2 isoform (APOE2) follows the endogenous mouse APOE and GFAP expression patterns in the brain. Human APOE2 is immunodetectable in glia and neuropil in developing and adult mutant mice. Cultured astrocytes from transgenic mice secrete APOE2 in lipoproteins that are similar in size to high density plasma lipoproteins (HDL). Detergent-soluble APOE2 protein levels in forebrain tissue from hemizygous mice and in adult human cortex tissue are similar.

Mice that are hemizygous or homozygous for the transgenic insert and homozygous for Apoetm1Unc are viable, normal in size, and do not display any gross physical or behavioral abnormalities.

Original:  J:93487 Sun Y, et al., Glial fibrillary acidic protein-apolipoprotein E (apoE) transgenic mice: astrocyte-specific expression and differing biological effects of astrocyte-secreted apoE3 and apoE4 lipoproteins. J Neurosci. 1998 May 1;18(9):3261-72
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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