Nodal^tm5Rob
Targeted Allele Detail

Summary

• Symbol: Nodal^tm5Rob
• Name: nodal growth differentiation factor; targeted mutation 5, Elizabeth J Robertson
• MGI ID: MGI:3056345
• Synonyms: Nodal^C
• Gene: Nodal Location: Chr10:61253751-61261117 bp, + strand Genetic Position: Chr10, 32.21 cM
• Alliance: Nodal^tm5Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93140
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed hygromycin cassette was inserted in intron 1 and a third loxP site was placed in intron 3. In vitro cre recombination removed the floxed hygromycin cassette from properly targeted ES cell clones. Exons 2 and 3 were then left flanked by loxP sites. Mice were crossed to a protamine deleter strain to confirm that the recombined allele had a null phenotype. (J:93140)

Expression

In Mice Carrying this Mutation:

23 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nodal Mutation: 41 strains or lines available

References

• Original: J:93140 Lu CC, et al., Multiple roles for Nodal in the epiblast of the mouse embryo in the establishment of anterior-posterior patterning. Dev Biol. 2004 Sep 1;273(1):149-59
• All: 4 reference(s)