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Transgene Detail
Symbol: Tg(CAG-EGFP)D4Nagy
Name: transgene insertion D4, Andras Nagy
MGI ID: MGI:3055027
Synonyms: D4/XEGFP, Tg(ACTB-EGFP)D4Nagy, Tg(CAG-EGFP)D4Nagy, Tg(CMV-GFP)1Jae, Tg(CMV-GFP)1Nagy, Tg(GFPX)4Nagy, XGFP, XGFP, X-GFP, Xp-GFP
Transgene: Tg(CAG-EGFP)D4Nagy  Location: unknown  Genetic Position: ChrX, Syntenic
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
Mutation detailsThe transgene contains the enhanced Green Fluorescent Protein driven by chicken beta-actin promoter and CMV intermediate early enhancer. Transgenic mice were generated by embryonic stem (ES) cell-mediated transgenesis. The construct was electroporated into R1 ES cells and random integration occurred on the X chromosome just distal of Timp and Syn1. (J:69995)
View phenotypes and curated references for all genotypes (concatenated display).
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Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Transgene expression is seen as fluorescence under exposure to a 488 nm light source. In transgenic mice the reporter may be detected as early as ~E2.75, as morula stage embryos begin to compact. Expression of GFP is spatiotemporally ubiquitous, though levels differ between different lineages (e.g., expression is slightly higher in the heart).

No discernible phenotype, other than green fluorescence, has been observed in any mice carrying this transgene.

Original:  J:69995 Hadjantonakis AK, et al., Non-invasive sexing of preimplantation stage mammalian embryos. Nat Genet. 1998 Jul;19(3):220-2
All:  41 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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