Cd22^tm2Tft
Targeted Allele Detail

Summary

• Symbol: Cd22^tm2Tft
• Name: CD22 antigen; targeted mutation 2, Thomas F Tedder
• MGI ID: MGI:3054883
• Synonyms: CD22AA
• Gene: Cd22 Location: Chr7:30564829-30579767 bp, - strand Genetic Position: Chr7, 19.26 cM
• Alliance: Cd22^tm2Tft page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92736
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: R130 and R137 codons were targeted to generate codons encoding alanine residues. A floxed neomycin resistance gene was inserted between exons 4 and 5. Crossing with mice expressing Cre in the germ line excised the floxed neo. Immunoblot and flow cytometry confirmed protein was expressed in spleen cell lysates from mutant mice. (J:92736)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd22 Mutation: 63 strains or lines available

References

• Original: J:92736 Poe JC, et al., CD22 regulates B lymphocyte function in vivo through both ligand-dependent and ligand-independent mechanisms. Nat Immunol. 2004 Oct;5(10):1078-87
• All: 2 reference(s)