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Magtm1Pop
Targeted Allele Detail
Nomenclature
Symbol: Magtm1Pop
Name: myelin-associated glycoprotein; targeted mutation 1, Brian Popko
MGI ID: MGI:3053091
Synonyms: L-Magtrun
Gene: Mag  Location: Chr7:30899176-30914873 bp, - strand  Genetic Position: Chr7, 19.26 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:46770
Parent Cell Line:  BK4 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA stop codon was introduced into the 13th exon as a result of a neo insertion. The final 49 amino acids of the long isoform should not be translated without altering expression of the short isoform. The putative protein of the long isoform is four amino acids shorter than the wild-type short isoform. Western blot revealed lack of protein of the wild-type long isoform in mutants. Protein of the short isoform was present, however at ~50% of wild-type levels. Northern blot revealed double bands in mutant mice, suggesting that the polyA site of the neo is being used to prematurely truncate the transcript, resulting in two bands with different 3' ends. (J:46770)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mag Mutation:  17 strains or lines available
References
Original:  J:46770 Fujita N, et al., The cytoplasmic domain of the large myelin-associated glycoprotein isoform is needed for proper CNS but not peripheral nervous system myelination. J Neurosci. 1998 Mar 15;18(6):1970-8
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/26/2020
MGI 6.15
The Jackson Laboratory