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Gja5tm1(Gja7)Hvr
Targeted Allele Detail
Summary
Symbol: Gja5tm1(Gja7)Hvr
Name: gap junction protein, alpha 5; targeted mutation 1, Harold van Rijen
MGI ID: MGI:3051866
Synonyms: Cx40KI45, Cx40KICx45
Gene: Gja5  Location: Chr3:96939718-96960950 bp, + strand  Genetic Position: Chr3, 42.04 cM
Alliance: Gja5tm1(Gja7)Hvr page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:92018
Parent Cell Line:  CK35 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Inserted expressed sequence, Null/knockout)
Mutation:    Insertion
 
Mutation detailsA targeting vector containing exon 3 (the coding exon) of Gjc1 (Gja7), a floxed neo, and exon 2 (the coding exon) of Gja5 with a loxP site near the 3' end was inserted immediately after the start site of the endogenous gene, within exon 2, so as to allow for the expression of Gjc1 in place of Gja5. The second amino acid of Gjc1 was mutated from a serine to a glycine. Mice transmitting the mutation through the germ line were crossed with either CAG-Cre or Cre-deleter mice to excise the floxed neo and most of exon 2 of Gja5. Southern blot and PCR confirmed recombination. RT-PCR, Northern blot and Western blot indicated exon 3 from Gjc1 was expressed in knock-in mice, while Gja5 was not expressed. (J:92018)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gja5 Mutation:  18 strains or lines available
References
Original:  J:92018 Alcolea S, et al., Replacement of connexin40 by connexin45 in the mouse: impact on cardiac electrical conduction. Circ Res. 2004 Jan 9;94(1):100-9
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory