Ace^tm5Keb
Targeted Allele Detail

Summary

• Symbol: Ace^tm5Keb
• Name: angiotensin I converting enzyme; targeted mutation 5, Kenneth E Bernstein
• MGI ID: MGI:3042130
• Synonyms: ACE.7
• Gene: Ace Location: Chr11:105858774-105880790 bp, + strand Genetic Position: Chr11, 68.84 cM
• Alliance: Ace^tm5Keb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89512
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: An allele carrying point mutations H395K and H399K in exon 8 was inserted via homologous recombination. Inactivation of the N-terminal catalytic site was confirmed using an N-terminal specific substrate. Expression of the mutant protein was shown to be equivalent to expression of the normal protein by western blot analysis. (J:89512)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace Mutation: 54 strains or lines available

Notes

J:163454 selected for the single renin gene locus found in C57BL/6 mice instead of the duplicated locus found in 129 mice.

References

• Original: J:89512 Fuchs S, et al., Role of the N-terminal catalytic domain of angiotensin-converting enzyme investigated by targeted inactivation in mice. J Biol Chem. 2004 Apr 16;279(16):15946-53
• All: 5 reference(s)