Tnfsf13^tm1Ash
Targeted Allele Detail

Summary

• Symbol: Tnfsf13^tm1Ash
• Name: tumor necrosis factor (ligand) superfamily, member 13; targeted mutation 1, Avi Ashkenazi
• MGI ID: MGI:3028906
• Synonyms: APRIL^-
• Gene: Tnfsf13 Location: Chr11:69573672-69576610 bp, - strand Genetic Position: Chr11, 42.86 cM, cytoband B4
• Alliance: Tnfsf13^tm1Ash page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:87680
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 2.5 kb genomic fragment, encompassing a portion of exon 1 and all of exons 2 through 6, was replaced with a neo cassette. The deletion was designed to eliminate both the secreted and transmembrane porteins produced by the endogenous locus. Tnfsf13 protein was undetected by FACS analysis of homozygous mutant splenocytes. Real-time RT-PCR indicated that the levels of Tnfsf12 and Senp3 transcript, produced by loci in close proximity to Tnfsf13, were not affected by the targeting event. (J:87680)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnfsf13 Mutation: 68 strains or lines available

References

• Original: J:87680 Varfolomeev E, et al., APRIL-deficient mice have normal immune system development. Mol Cell Biol. 2004 Feb;24(3):997-1006
• All: 8 reference(s)