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Rorctm2Litt
Targeted Allele Detail
Nomenclature
Symbol: Rorctm2Litt
Name: RAR-related orphan receptor gamma; targeted mutation 2, Dan R Littman
MGI ID: MGI:3026635
Synonyms: Roc(gt)gfp, Rorc(gammat)-, Rorc(gammat)-EGFP, RorcGFP, RORgammat, RORgammat-EGFP
Gene: Rorc  Location: Chr3:94372794-94398276 bp, + strand  Genetic Position: Chr3, 40.56 cM, cytoband F2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:87395
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsAn isoform-specific disruption was generated by inserting a cassette containing eGFP downstream of the start codon of exon 1gammat. The targeted exon is the third exon of the gene, serves as an alternative first exon for the gammat transcript, and is not included in the gamma transcript. RT-PCR analysis of hepatic RNA obtained from homozygous mutant mice identified gamma transcript but not the targted gammat transcript. The eGFP sequence included a stop codon but no splice sites or polyadenylation signals. A floxed neo cassette included in the targeting vector was excised from the germline via cre-mediated recombination. (J:87395)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Rorc Mutation:  25 strains or lines available
References
Original:  J:87395 Eberl G, et al., An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol. 2004 Jan;5(1):64-73
All:  117 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
12/03/2019
MGI 6.14
The Jackson Laboratory