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Sftpdtm2Haw
Targeted Allele Detail
Nomenclature
Symbol: Sftpdtm2Haw
Name: surfactant associated protein D; targeted mutation 2, Samuel Hawgood
MGI ID: MGI:2677969
Synonyms: SP-AD(-)
Gene: Sftpd  Location: Chr14:41172212-41185198 bp, - strand  Genetic Position: Chr14, 22.36 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:86022
Parent Cell Line:  CB1-4 (ES Cell)
Strain of Origin:  C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsUsing ES cells in which a correctly targeted modification of the Sftpa gene had already been created, exon 2 along with the translation start site, was replaced with a PGK-neomycin cassette. Northern blot analysis demonstrated the absence of any Sftpa mRNA in any doubly homozygous mice as well as the absence of full length Sftpd mRNA. A small amount of truncated mRNA for Sftpd lacking the translation start site was seen. Western blot analysis confirmed that no protein products were produced. (J:86022)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sftpd Mutation:  9 strains or lines available
Notes
This allele was generated in ES cells containing Sftpa1tm2Haw.
References
Original:  J:86022 Hawgood S, et al., Sequential targeted deficiency of SP-A and -D leads to progressive alveolar lipoproteinosis and emphysema. Am J Physiol Lung Cell Mol Physiol. 2002 Nov;283(5):L1002-10
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/12/2017
MGI 6.10
The Jackson Laboratory