Itga2b^{tm1(GFP/cre)Emam}
Targeted Allele Detail

Summary

• Symbol: Itga2b^{tm1(GFP/cre)Emam}
• Name: integrin alpha 2b; targeted mutation 1, Nikla R Emambokus
• MGI ID: MGI:2670210
• Synonyms: gpIIb^-, gpIIb^GFPCre
• Gene: Itga2b Location: Chr11:102344123-102360709 bp, - strand Genetic Position: Chr11, 66.29 cM
• Alliance: Itga2b^{tm1(GFP/cre)Emam} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84564
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutation: Insertion
• Itga2b^{tm1(GFP/cre)Emam} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Itga2b (MGI:96601)

• Mutation details: The endogenous locus was disrupted by homologous recombination of a targeting vector containing sequence encoding a GFP-cre fusion protein followed by an FRT-flanked PGK-neo cassette. While expression of cre recombinase was detected in (and limited to) the megakaryocyte lineage by Western blot analysis, GFP fluorescence was not observed. Endogenous protein was undetected by either flow cytometry or Western blot analysis of homozygous mutant bone marrow cells. (J:84564)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Itga2b (mouse) Summary of all recombinase alleles driven by Itga2b. Alliance mouse Itga2b gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itga2b Mutation: 45 strains or lines available

References

• Original: J:84564 Emambokus NR, et al., The glycoprotein IIb molecule is expressed on early murine hematopoietic progenitors and regulates their numbers in sites of hematopoiesis. Immunity. 2003 Jul;19(1):33-45
• All: 14 reference(s)