Cd81^tm1Tmiy
Targeted Allele Detail

Summary

• Symbol: Cd81^tm1Tmiy
• Name: CD81 antigen; targeted mutation 1, Toru Miyazaki
• MGI ID: MGI:2665072
• Synonyms: CD81^-
• Gene: Cd81 Location: Chr7:142606487-142621667 bp, + strand Genetic Position: Chr7, 88.1 cM
• Alliance: Cd81^tm1Tmiy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:41830
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 through 7 and part of exon 8 (including the TGA stop codon) were replaced with a Neomycin resistance gene. These exons encode the second extracellular loop, the second through fourth membrane spanning domains and the entire C-terminal intracellular domain. Absence of gene expression was established by Northern blot analysis and RT-PCR and absence of protein was confirmed by cytofluorometric analysis of splenic B cells. (J:41830)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd81 Mutation: 21 strains or lines available

References

• Original: J:41830 Miyazaki T, et al., Normal development but differentially altered proliferative responses of lymphocytes in mice lacking CD81. EMBO J. 1997 Jul 16;16(14):4217-25
• All: 8 reference(s)