Igf2r^tm2Wag
Targeted Allele Detail

Summary

• Symbol: Igf2r^tm2Wag
• Name: insulin-like growth factor 2 receptor; targeted mutation 2, Erwin F Wagner
• MGI ID: MGI:2445841
• Synonyms: R2Delta
• Gene: Igf2r Location: Chr17:12901293-12988551 bp, - strand Genetic Position: Chr17, 8.64 cM, cytoband A-C
• Alliance: Igf2r^tm2Wag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68721
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The endogenous allele was released from imprint-mediated regulation by deleting the CpG rich sequences of region 2 using molecular techniques. A 3.7 kb fragment was replaced with a floxed hygro cassette which was subsequently excised in vitro via cre mediated recombination. Slot blot and RNAse protection assay analyses showed normal transcript to be produced from the targeted locus. (J:68721)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igf2r Mutation: 98 strains or lines available

References

• Original: J:68721 Wutz A, et al., Non-imprinted Igf2r expression decreases growth and rescues the Tme mutation in mice. Development. 2001 May;128(10):1881-7
• All: 3 reference(s)