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Krastm4Tyj
Targeted Allele Detail
Nomenclature
Symbol: Krastm4Tyj
Name: Kirsten rat sarcoma viral oncogene homolog; targeted mutation 4, Tyler Jacks
MGI ID: MGI:2429948
Synonyms: caKRas, KR, Kras2tm14Tyj, Kras2tm4Tyj, KrasG12D, K-RasG12D, K-Ras(G12D)fl, KrasLox, K-rasLSL, KrasLSL-G12D, LSL-KrasG12D, LSL-K-rasG12D, LSL-K-ras G12D, LSL-KrasG12D, LSL-Kras G12D
Gene: Kras  Location: Chr6:145216699-145250239 bp, - strand  Genetic Position: Chr6, 77.37 cM, cytoband G2
Pancreatic tumors in Krastm4Tyj/Krastm4Tyj Trim33tm1Los/Trim33tm1Los Tg(Pdx1-cre)89.1Dam/0 and Krastm4Tyj/Krastm4Tyj Cdkn2atm4Rdp/Cdkn2atm4Rdp Tg(Pdx1-cre)89.1Dam/0 mice

Show the 4 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:73445
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsBy homologous recombination in ES cells, the Kras2 locus was targeted with a cassette containing an oncogenic form of the KRAS2 protein in which the glycine at position 12 had been substituted with a an aspartic acid. A loxP flanked stop codon was included upstream of the inserted Kras2 sequence, such that the mutant transcript would be expressed only after cre-mediated recombination. (J:73445)
Phenotypes
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Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Kras Mutation:  32 strains or lines available
Notes
Phenotypic Similarity to Human Syndrome: Intrahepatic Cholangiocarcinoma (J:184949).

Phenotypic Similarity to Human Syndrome: Granulosa cell tumor (GCT) J:186144

Phenotypic Similarity to Human Syndrome: Granulosa cell tumor of the testis (GCTT) J:186144

Phenotypic Similarity to Human Syndrome: Squamous Cell Carcinoma J:203922 in double Kras and Smad4 mutants.

Phenotypic Similarity to Human Syndrome: Soft Tissue Sarcoma J:125101 in double Kras and Trp53 mutants and in double Kras and Cdkn2a mutants.

Phenotypic Similarity to Human Syndrome: Soft Tissue Sarcoma, Undifferentiated Pleomorphic Sarcoma/Malignant Fibrous Histiocytoma J:155389, J:204376 in double Kras and Trp53 mutants.

Phenotypic Similarity to Human Syndrome: Non-Small-Cell Lung Carcinoma J:124682 in double Kras and Stk11 mutants.

Phenotypic Similarity to Human Syndrome: HPV-Positive Oral Cancer J:210533 .

Phenotypic Similarity to Human Syndrome: Skin Cancer (Squamous Cell Carcinoma) J:172048 in double Kras and Trp53 conditional(Tg(KRT14-cre/ERT)20Efu) mutants.

References
Original:  J:73445 Jackson EL, et al., Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Genes Dev. 2001 Dec 15;15(24):3243-8
All:  581 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/27/2016
MGI 6.05
The Jackson Laboratory