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Prnptm2Edin
Targeted Allele Detail
Nomenclature
Symbol: Prnptm2Edin
Name: prion protein; targeted mutation 2, Edinburgh University
MGI ID: MGI:2387688
Synonyms: mPrP0/0, NPU, PrnpEdbg, Prnp- Edinburgh, PrP-
Gene: Prnp  Location: Chr2:131909928-131938429 bp, + strand  Genetic Position: Chr2, 64.07 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:20398
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsThe gene was disrupted by insertion of a neomycin resistance cassette (under the control of a mouse metallothionein promoter) into exon 3. Northern blot analysis of brain mRNA from homozygous mutant animals did not detect gene transcript, but detected the neo transcript. Western blot of brain membrane fractions from homozygous animals further confirmed the null allele. (J:20398)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Prnp Mutation:  82 strains or lines available
References
Original:  J:20398 Manson JC, et al., 129/Ola mice carrying a null mutation in PrP that abolishes mRNA production are developmentally normal. Mol Neurobiol. 1994 Apr-Jun;8(2-3):121-7
All:  46 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
02/25/2015
MGI 5.21
The Jackson Laboratory