Ikbkg^tm1Mpa
Targeted Allele Detail

Summary

• Symbol: Ikbkg^tm1Mpa
• Name: inhibitor of kappaB kinase gamma; targeted mutation 1, Manolis Pasparakis
• MGI ID: MGI:2386324
• Synonyms: Nemo^D, Nemo^hko
• Gene: Ikbkg Location: ChrX:73436896-73497460 bp, + strand Genetic Position: ChrX, 38.0 cM
• Alliance: Ikbkg^tm1Mpa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63055
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Flanking loxP sites were introduced to exon 2 and an FRT-flanked neomycin resistance cassette was inserted into the downstream intron via homologous recombination. Deletion of exon 2 and the neo cassette was carried out by transient expression of cre and flp recombinases in correctly targeted ES cells. Western blot analysis of mouse embyonic fibroblasts cultured from E12.5 homozygous mutant embryos confirmed the null allele. It was also shown that the targeted mutation did not interfere with neighboring gene G6pdx. (J:63055)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ikbkg Mutation: 15 strains or lines available

References

• Original: J:63055 Schmidt-Supprian M, et al., NEMO/IKK gamma-deficient mice model incontinentia pigmenti. Mol Cell. 2000 Jun;5(6):981-92
• All: 5 reference(s)