Hras^tm1Esn
Targeted Allele Detail

Summary

• Symbol: Hras^tm1Esn
• Name: Harvey rat sarcoma virus oncogene; targeted mutation 1, Eugenio Santos
• MGI ID: MGI:2385945
• Synonyms: H-ras^-
• Gene: Hras Location: Chr7:140770839-140773938 bp, - strand Genetic Position: Chr7, 86.48 cM
• Alliance: Hras^tm1Esn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67374
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1, 2, and 3 were replaced by a neomycin selection cassette via homologous recombination. Homozygous mutant mice were shown to lack transcript by RT-PCR analysis. Western blot analysis showed that the mutant allele did not lead to the produciton of protein. (J:67374)

Tumor Data

List all tumor models in MMHCdb carrying Hras^tm1Esn

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hras Mutation: 30 strains or lines available

References

• Original: J:67374 Esteban LM, et al., Targeted genomic disruption of H-ras and N-ras, individually or in combination, reveals the dispensability of both loci for mouse growth and development. Mol Cell Biol. 2001 Mar;21(5):1444-52
• All: 17 reference(s)