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Cckbrtm1Tom
Targeted Allele Detail
Summary
Symbol: Cckbrtm1Tom
Name: cholecystokinin B receptor; targeted mutation 1, Toshimitsu Matsui
MGI ID: MGI:2384535
Synonyms: CCK2-, CCK-2R-, CCK2R-, CCK2RKO, CCKBR-
Gene: Cckbr  Location: Chr7:105074882-105085546 bp, + strand  Genetic Position: Chr7, 55.86 cM
Alliance: Cckbrtm1Tom page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:35876
Parent Cell Line:  J1 (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsPart of exon 2 and exons 3-5 were replaced with a lacZ-PGK-neo cassette via homologous recombination resulting in deletion of the majority of 7 transmembrane regions after amino acid 108. Absence of gene expression was demonstrated by Northern blot analysis of cerebral cortex, basal ganglion, stomach, and pancreas mRNA from homozygous mutant animals using a 2.3 kb cDNA fragment as the probe. Absence of functional receptor protein was confirmed via binding assays using brain cell membrane preparations from homozygous mutants. (J:35876)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cckbr Mutation:  15 strains or lines available
References
Original:  J:35876 Nagata A, et al., G protein-coupled cholecystokinin-B/gastrin receptors are responsible for physiological cell growth of the stomach mucosa in vivo. Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11825-30
All:  35 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/22/2022
MGI 6.22
The Jackson Laboratory