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Transgene Detail
Symbol: Tg(EEF1A1-SHC1*)1Ravi
Name: transgene insertion 1, Kodi S Ravichandran
MGI ID: MGI:2384188
Synonyms: ShcFFF, STOPfloxShcFFF
Transgene: Tg(EEF1A1-SHC1*)1Ravi  Location: unknown  
Strain of Origin:  129
Transgene Type:    Transgenic (Conditional ready, Dominant negative, Inserted expressed sequence, Reporter)
Mutation:    Insertion
Tg(EEF1A1-SHC1*)1Ravi expresses 1 gene
Mutation detailsA construct was generated in which a human EF-1alpha promoter was separated from a modified human SHC1 cDNA fused to an IRES-GFP by a floxed stop codon. The floxed stop codon allowed transcriptional control via the expression of cre recombinase. SHC1 codons encoding three tyrosine residues (positions 239, 240, 317) that are phosphorylated after cross linking were modified to encode phenylalanine. This creates a dominant-negative protein in certain signaling pathways. Breeding of mice carrying this allele with transgenic mice expressing cre resulted in the expression of the modified SHC1, though not GFP. (J:78180)
Inheritance:    Dominant
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:78180 Zhang L, et al., A nonredundant role for the adapter protein Shc in thymic T cell development. Nat Immunol. 2002 Aug;3(8):749-55
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory