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Targeted Allele Detail
Symbol: Nodaltm1.2Rob
Name: nodal; targeted mutation 1.2, Elizabeth J Robertson
MGI ID: MGI:2181674
Synonyms: delta100.lacZ, delta100 nodal.lacZ
Gene: Nodal  Location: Chr10:61417972-61425338 bp, + strand  Genetic Position: Chr10, 32.21 cM
Germline Transmission:  Earliest citation of germline transmission: J:56145
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsBy homologous recombination in ES cells heterozygous for the Nodaltm1Rob allele, 100 bp of the ASE enhancer region (including the two 10 bp binding sites) was replaced by a loxP-flanked hygromycin selection cassette in cis to the original mutation. Transient expression of Cre recombinase in targeted ES cells resulted in excision of the selection cassette, leaving a single loxP site in place of the enhancer sequences. (J:56145)
View phenotypes for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nodal Mutation:  18 strains or lines available
Original:  J:56145 Norris DP, et al., Asymmetric and node-specific nodal expression patterns are controlled by two distinct cis-acting regulatory elements. Genes Dev. 1999 Jun 15;13(12):1575-88
All:  5 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.06
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