Tg(Lck)1Rmp
Transgene Detail

Summary

• Symbol: Tg(Lck)1Rmp
• Name: transgene insertion 1, Roger M Perlmutter
• MGI ID: MGI:2181670
• Synonyms: lckR273, p56^lckR273
• Transgene: Tg(Lck)1Rmp Location: unknown
• Alliance: Tg(Lck)1Rmp page

Transgene origin

• Strain of Origin: (C57BL/6J x DBA/2J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Lck)1Rmp expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Lck (MGI:96756)

• Mutation details: A catalytically inactive allele of the gene was introduced via transgene insertion. The transgene includes exons 1-12, 240 bp of endogenous promoter sequence, a lysine to arginine substitution at codon 273 (K273R), and a fusion to the 3' untranslated region of the human growth hormone gene. To maximize transgene expression, 125 bp of exon 1, which encodes 5' untranslated sequences known to reduce translational efficiency, were removed. The endogenous promoter was used to direct transgene expression in the thymus. (J:76887)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:76887 Levin SD, et al., A dominant-negative transgene defines a role for p56lck in thymopoiesis. EMBO J. 1993 Apr;12(4):1671-80
• All: 2 reference(s)