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Targeted Allele Detail
Symbol: Nodaltm2Rob
Name: nodal; targeted mutation 2, Elizabeth J Robertson
MGI ID: MGI:2181583
Synonyms: NodalD600, nodaldelta600
Gene: Nodal  Location: Chr10:61417972-61425338 bp, + strand  Genetic Position: Chr10, 32.21 cM
Germline Transmission:  Earliest citation of germline transmission: J:56145
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsBy homologous recombination in ES cells heterozygous for the Nodaltm1Rob allele, the entire 600 bp ASE enhancer region was replaced by a loxP-flanked hygromycin selection cassette in trans to the original mutation. Transient expression of Cre recombinase in targeted ES cells resulted in excision of the selection cassette, leaving a single loxP site in place of the enhancer sequences. Subsequent matings of mice carrying this allele resulted in segregation from the Nodaltm1Rob allele. (J:77125)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 66 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nodal Mutation:  24 strains or lines available
Original:  J:56145 Norris DP, et al., Asymmetric and node-specific nodal expression patterns are controlled by two distinct cis-acting regulatory elements. Genes Dev. 1999 Jun 15;13(12):1575-88
All:  7 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.11
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