Ctla4^tm1Shr
Targeted Allele Detail

Summary

• Symbol: Ctla4^tm1Shr
• Name: cytotoxic T-lymphocyte-associated protein 4; targeted mutation 1, Arlene H Sharpe
• MGI ID: MGI:2180668
• Synonyms: Ctla4^-, CTLA-4^-
• Gene: Ctla4 Location: Chr1:60948184-60954991 bp, + strand Genetic Position: Chr1, 30.58 cM, cytoband C
• Alliance: Ctla4^tm1Shr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:30393
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of a PGK-neo cassette replaced a 1.3 kb region containing exon 2 and a portion of exon 3. The deleted region encoded a IgV and part of a transmembrane domain. Fluorescence-activated cell sorting (FACS) analysis showed an absence of protein in splenocytes isolated from homozygous mutant mice. (J:30393)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Ctla4 Mutation: 53 strains or lines available

References

• Original: J:30393 Tivol EA, et al., Loss of CTLA-4 leads to massive lymphoproliferation and fatal multiorgan tissue destruction, revealing a critical negative regulatory role of CTLA-4. Immunity. 1995 Nov;3(5):541-7
• All: 44 reference(s)