Ckmt2^tm1Bew
Targeted Allele Detail

Summary

• Symbol: Ckmt2^tm1Bew
• Name: creatine kinase, mitochondrial 2; targeted mutation 1, Be Wieringa
• MGI ID: MGI:2180507
• Synonyms: Mib-CK-, Mito-CK^-, MtCK^-, ScCKmit-
• Gene: Ckmt2 Location: Chr13:92001510-92025001 bp, - strand Genetic Position: Chr13, 47.43 cM
• Alliance: Ckmt2^tm1Bew page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76886
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 400 bp of the gene including part of exon 2, exon 3, and the translation initiation codon was replaced with a hygromycin resistance cassette via homologous recombination. Northern blot analysis detected mutant transcripts in cardiac and skeletal muscle of homozygous mutant animals, but Western blot analysis of the same tissues demonstrated the absence of protein product. (J:76886)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ckmt2 Mutation: 24 strains or lines available

References

• Original: J:76886 Steeghs K, et al., Use of gene targeting for compromising energy homeostasis in neuro-muscular tissues: the role of sarcomeric mitochondrial creatine kinase. J Neurosci Methods. 1997 Jan;71(1):29-41
• All: 22 reference(s)