Poll^tm1Crey
Targeted Allele Detail

Summary

• Symbol: Poll^tm1Crey
• Name: polymerase (DNA directed), lambda; targeted mutation 1, Claude-Agnes Reynaud
• MGI ID: MGI:2179232
• Synonyms: pol lambda-
• Gene: Poll Location: Chr19:45540714-45548970 bp, - strand Genetic Position: Chr19, 38.75 cM
• Alliance: Poll^tm1Crey page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75927
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 5-7 were replaced with a neomycin resistance gene via homologous recombination resulting in the loss of the DNA polymerase catalytic site and HhH domain. RT-PCR of spleen B cells from homozygous mutant animals failed to detect gene expression. (J:75927)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Poll Mutation: 35 strains or lines available

References

• Original: J:75927 Bertocci B, et al., Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation. J Immunol. 2002 Apr 15;168(8):3702-6
• All: 4 reference(s)