Lyn^tm1Mhi
Targeted Allele Detail

Summary

• Symbol: Lyn^tm1Mhi
• Name: LYN proto-oncogene, Src family tyrosine kinase; targeted mutation 1, Margaret Hibbs
• MGI ID: MGI:2157016
• Synonyms: Lyn^up, Lyn^Y508F
• Gene: Lyn Location: Chr4:3676865-3791612 bp, + strand Genetic Position: Chr4, 2.05 cM
• Alliance: Lyn^tm1Mhi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72269
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Insertion of an IRES-neomycin resistance cassette into exon 13 introduced a Tyr508Phe point mutation. Northern blot analysis of bone marrow macrophages detected expression of a truncated message in homozygous mutant mice. Immunoblot analysis detected reduced levels of protein in bone marrow macrophages from homozygous mutant mice. (J:72269)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Lyn Mutation: 64 strains or lines available

References

• Original: J:72269 Harder KW, et al., Gain- and loss-of-function lyn mutant mice define a critical inhibitory role for lyn in the myeloid lineage. Immunity. 2001 Oct;15(4):603-15
• All: 8 reference(s)