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Epha4tm1Pch
Targeted Allele Detail
Nomenclature
Symbol: Epha4tm1Pch
Name: Eph receptor A4; targeted mutation 1, Patrick Charnay
MGI ID: MGI:2149729
Synonyms: EphA4-, EphA4KO, Epha4lacZ
Gene: Epha4  Location: Chr1:77367185-77515088 bp, - strand  Genetic Position: Chr1, 39.55 cM, cytoband C1-C5
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:62985
Parent Cell Line:  CK35 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExon 1, which encodes the first 30 amino acids, and part of the first intron were replaced by a lacZ-PGK-neomycin resistance cassette. The lacZ-coding sequence was placed immediately downstream of and in frame with the initiating codon. In addition, a frameshift mutation was introduced into exon 3. Western blot analysis did not detect protein in heads of homozygous mutant E14.5 embryos. Immunohistochemistry did not detect protein in the brachial region of homozygous mutant E11.5 embryos. (J:62985)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 37 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Epha4 Mutation:  9 strains or lines available
References
Original:  J:62985 Helmbacher F, et al., Targeting of the EphA4 tyrosine kinase receptor affects dorsal/ventral pathfinding of limb motor axons. Development. 2000 Aug;127(15):3313-24
All:  21 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/24/2020
MGI 6.15
The Jackson Laboratory