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Gt(ROSA)26Sortm1Sho
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm1Sho
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Stuart Orkin
MGI ID: MGI:1861931
Synonyms: gtROSA, R26-beta-galfl-STOP, R26fslz, R26R, R26Rosa-lox-Stop-lox-LacZ, R26RstoplacZ, Rosa26fsLz, ROSA26-loxP-stoploxP-beta-geo, ROSA26R-LacZ reporter, ROSA26-stop-lacZ
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:64291
Parent Cell Line:  AK7 (ES Cell)
Strain of Origin:  129S4/SvJaeSor
Mutation
description
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
 
Mutation detailsA targeting vector was designed to insert floxed (flanked by loxP) phosphoglycerate kinase-1-cytosine deaminase/phosphoglycerate kinase-1-puromycin (PGK-CD/PGK-Puro) and stop sequences between the splicing acceptor (SA) and the beta-gal-neomycin phosphotransferase fusion gene (beta-geo) sequences of the proviral ROSA 26 locus. Correctly targeted, this resulted in ES cells that were Puro resistant but G418 susceptible, and did not express beta-gal. (J:64291)
Inheritance:    Not Specified
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 1 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  534 strains or lines available
Notes
Mice carrying this mutation are considered to be reporter strains, with successful Cre excision indicated by beta-gal expression in Cre-expressing tissues.
References
Original:  J:64291 Mao X, et al., Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5037-42
All:  97 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
07/29/2020
MGI 6.15
The Jackson Laboratory