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Targeted Allele Detail
Symbol: Gt(ROSA)26Sortm1Sho
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Stuart Orkin
MGI ID: MGI:1861931
Synonyms: gtROSA, R26-beta-galfl-STOP, R26fslz, R26R, R26Rosa-lox-Stop-lox-LacZ, R26RstoplacZ, Rosa26fsLz, ROSA26-loxP-stoploxP-beta-geo, ROSA26R-LacZ reporter, ROSA26-stop-lacZ
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Germline Transmission:  Earliest citation of germline transmission: J:64291
Parent Cell Line:  AK7 (ES Cell)
Strain of Origin:  129S4/SvJaeSor
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
Mutation detailsA targeting vector was designed to insert floxed (flanked by loxP) phosphoglycerate kinase-1-cytosine deaminase/phosphoglycerate kinase-1-puromycin (PGK-CD/PGK-Puro) and stop sequences between the splicing acceptor (SA) and the beta-gal-neomycin phosphotransferase fusion gene (beta-geo) sequences of the proviral ROSA 26 locus. Correctly targeted, this resulted in ES cells that were Puro resistant but G418 susceptible, and did not express beta-gal. (J:64291)
Inheritance:    Not Specified
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  432 strains or lines available
Mice carrying this mutation are considered to be reporter strains, with successful Cre excision indicated by beta-gal expression in Cre-expressing tissues.
Original:  J:64291 Mao X, et al., Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5037-42
All:  92 reference(s)

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last database update
MGI 6.13
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