Rbl1^tm1Mru
Targeted Allele Detail

Summary

• Symbol: Rbl1^tm1Mru
• Name: RB transcriptional corepressor like 1; targeted mutation 1, Michael Rudnicki
• MGI ID: MGI:1857798
• Synonyms: p107-
• Gene: Rbl1 Location: Chr2:156987813-157046454 bp, - strand Genetic Position: Chr2, 78.05 cM
• Alliance: Rbl1^tm1Mru page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51060
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The Rbl1 gene was disrupted by insertion of a PGK neomycin resistance cassette into exon 4 immediately downstream of the sequence encoding amino acid 165. Northern analysis of embryonic fibroblast confluent cultures detected a slightly truncated Rbl1 transcript in homozygous mutant E14.5 embryos. The authors propose that the truncated transcript would be initiated from the PGK promoter. Immunoblot analysis using antisera reactive with the carboxyl-terminal 18 amino acids of RBL1 did not detect protein in homozygous mutant embryos. (J:51060)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rbl1 Mutation: 60 strains or lines available

References

• Original: J:51060 LeCouter JE, et al., Strain-dependent myeloid hyperplasia, growth deficiency, and accelerated cell cycle in mice lacking the Rb-related p107 gene. Mol Cell Biol. 1998 Dec;18(12):7455-65
• All: 10 reference(s)