Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tgs1tm1.2Jkr
Name:	trimethylguanosine synthase 1; targeted mutation 1.2, Janardan K Reddy
MGI ID:	MGI:5444606
Synonyms:	PIMT-, PIMTnull
Gene:	Tgs1  Location: Chr4:3574875-3616619 bp, + strand  Genetic Position: Chr4, 1.99 cM, cytoband A1
Alliance:	Tgs1tm1.2Jkr page

Germline Transmission:	Earliest citation of germline transmission: J:189008
Parent Cell Line:	HM-1 (ES Cell)
Strain of Origin:	129P2/OlaHsd-Hprt1b-m3

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: An FRT flanked neo cassette and loxP site were inserted upstream of exon 3 and a loxP site was inserted downstream of exon 4. Flp and cre mediated recombination removed the neo cassette and exons 3 and 4. Immunostaining analysis confirmed the absence of protein expression on homozygous blastocysts at E3.5. (J:189008)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	26 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tgs1 Mutation:	31 strains or lines available

Original:	J:189008 Jia Y, et al., Early embryonic lethality of mice with disrupted transcription cofactor PIMT/NCOA6IP/Tgs1 gene. Mech Dev. 2012 Sep;129(9-12):193-207
All:	1 reference(s)