Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Top1tm1Unc
Name:	topoisomerase (DNA) I; targeted mutation 1, University of North Carolina
MGI ID:	MGI:2678314
Synonyms:	Top-1 -
Gene:	Top1  Location: Chr2:160487901-160564684 bp, + strand  Genetic Position: Chr2, 80.96 cM, cytoband H2-3
Alliance:	Top1tm1Unc page

Germline Transmission:	Earliest citation of germline transmission: J:36546
Parent Cell Line:	BK4 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A neomycin selection cassette replaced a genomic fragment containing exon 15. Western blot analysis demonstrated that a 45% reduction of encoded protein levels was present in cells derived from heterozygous mice. Cytological analysis of early homozygous mutant embryos demonstrated that no detectable protein was produced from this allele. (J:36546)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Top1 Mutation:	210 strains or lines available

Original:	J:36546 Morham SG, et al., Targeted disruption of the mouse topoisomerase I gene by camptothecin selection. Mol Cell Biol. 1996 Dec;16(12):6804-9
All:	1 reference(s)