Probe:	Zimp10 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Embryos were re-fixed with 0.2% glutaraldehyde/ 4% PFA prior to hybridization.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	5A	Expression is in the epiblast, but is more abundant in the anterior portion.
TS11: allantois	Present		5A
TS11: embryo mesoderm	Present		5A

Structure	Level	Pattern	Image	Note
TS13: allantois	Present		5B
TS13: dorsal aorta	Present		5B
TS13: neural ectoderm	Present		5B
TS13: notochord	Present		5B

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		5C
TS13: paraxial mesenchyme	Present		5C

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum	Present		5F
TS13: neural ectoderm	Present		5F
TS13: paraxial mesenchyme	Present		5F

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		5G	There is a broad distribution throughout the embryo.
TS15: branchial arch	Present		5G
TS15: central nervous system	Present		5G
TS15: optic vesicle	Present		5G
TS15: otocyst	Present		5G
TS15: primitive ventricle	Absent		5G
TS15: tail	Absent		5G

Structure	Level	Pattern	Image	Note
TS15: branchial arch artery	Strong		5H
TS15: future brain	Strong		5H
TS15: somite	Strong		5H
TS15: umbilical vein	Strong		5H
TS15: surface ectoderm	Strong	Regionally restricted	5H	Expression is in the epithelium around the head.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		6A
TS17: forelimb bud	Present		6A
TS17: hindlimb bud	Present		6A
TS17: optic cup	Present		6A
TS17: otocyst	Present		6A
TS17: somite	Present		6A
TS17: telencephalon	Present		6A

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Uniform	6B
TS17: dorsal aorta	Absent		6B

Structure	Level	Pattern	Image	Note
TS19: brain	Present		6C
TS19: limb	Present		6C
TS19: tail	Present		6C

Structure	Level	Pattern	Image	Note
TS20: limb	Present		6D
TS20: tail	Present		6D

Structure	Level	Pattern	Image	Note
TS16: limb mesenchyme	Present	Regionally restricted	7A	There is broad expression in the mesenchyme and a discrete gradient of expression was observed in the posterior part of the limb.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted	7B	Expression is in the posterior mesenchyme.
TS17: apical ectodermal ridge	Absent		7B

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	7C	Expression is in the posterior mesenchyme in the prospective metatarsal region and an additional expression forming two stripes in the proximal mesenchyme close to the prospective fibula and tibia.

Structure	Level	Pattern	Image	Note
TS21: hindlimb interdigital region	Present	Regionally restricted	7D	Expression is in the proximal interdigital region up to the metatarsal region.

Structure	Level	Pattern	Image	Note
TS22: limb digit	Present	Regionally restricted	7E	Expression is in the interdigits to the digits at the forming phalangeal joints.
TS22: limb interdigital region	Absent		7E

Structure	Level	Pattern	Image	Note
TS20: fronto-nasal process	Trace			This expression was only seen after prolonged exposure times.
TS20: vibrissa	Trace			This expression was only seen after prolonged exposure times.

Structure	Level	Pattern	Image	Note
TS21: brain	Present

Structure	Level	Pattern	Image	Note
TS24: limb digit	Present	Regionally restricted		Expression is in the interdigits to the digits at the forming phalangeal joints.
TS24: limb interdigital region	Absent