Probe:	Dact1 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The expression patterns were validated with a probe that corresponds to nucleotides 316-692.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present
TS11: neural ectoderm	Weak

Structure	Level	Pattern	Image	Note
TS14: 1st arch artery	Present		2A
TS14: 2nd arch artery	Present		2A
TS14: Rathke's pouch	Present		2A
TS14: head mesenchyme	Present		2A
TS14: septum transversum	Present		2A
TS14: dorsal aorta	Present		2A	Including the aortic sac.
TS14: trunk unsegmented mesenchyme	Present	Graded	2A	The high expressing zone is in the caudal presomitic mesoderm.
TS14: trunk somite	Present	Graded	2A	There is a strong caudal to rostral gradient that inversely correlates with the developmental age of somites: highest expression in the most recently formed somite.

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present	Regionally restricted	2A inset	In younger somites, expression is ventromedially and along both the rostral and caudal somite walls, in older somites it is more ventromedial and caudal.

Structure	Level	Pattern	Image	Note
TS17: tail somite	Strong		2D
TS17: tail unsegmented mesenchyme	Strong		2D
TS17: tail mesenchyme	Weak	Regionally restricted	2D	Expression is in the ventral mesoderm.
TS17: forelimb bud mesenchyme	Present	Graded	2D	Expression is in a proximal (low) to apical (high) gradient.
TS17: hindlimb bud mesenchyme	Present	Graded	2D	Expression is in a proximal (low) to apical (high) gradient.

Structure	Level	Pattern	Image	Note
TS14: dorsal aorta	Present		2G
TS14: nephrogenic cord	Present		2G
TS14: foregut	Present	Regionally restricted	2G	Expression is in the mesenchymal outer walls of the foregut.
TS14: embryo	Present	Regionally restricted	2G	Expression is in the ventral mesentery.

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present	Regionally restricted	2I	In younger somites, expression is ventromedially and along both the rostral and caudal somite walls, in older somites it is more ventromedial and caudal.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Graded	2J	Expression is in a proximal (low) to apical (high) gradient.
TS17: hindlimb bud mesenchyme	Present	Graded	2J	Expression is in a proximal (low) to apical (high) gradient.
TS17: lung mesenchyme	Present	Regionally restricted	2J	Expression is in the left and right main bronchi.
TS17: trunk sclerotome	Present	Regionally restricted	2J	Expression is in the sclerotome derived from the ventral somite.
TS17: neural tube lateral wall	Present	Regionally restricted	2J	Expression is in post-mitotic neurons in the differentiating motor pools of the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS17: tail unsegmented mesenchyme	Strong		2L
TS17: tail somite	Strong	Regionally restricted	2L	Expression is in the caudal somites.

Structure	Level	Pattern	Image	Note
TS17: branchial arch mesenchyme	Weak

Structure	Level	Pattern	Image	Note
TS22: brain	Present		3A
TS22: cerebellum	Present		3A
TS22: pons	Present		3A
TS22: spinal cord	Present		3A
TS22: hindbrain	Present	Regionally restricted	3A	Expression is in cerebellar precursors near the midbrain?hindbrain junction.
TS22: midbrain	Present	Regionally restricted	3A	Expression is in cerebellar precursors near the midbrain?hindbrain junction.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present	Graded	3D	Expression is in a ventral (high) to dorsal (low) gradient.
TS22: cortical plate	Present	Graded	3D	Expression is in a rostroventral (low) to caudodorsal (high) gradient.
TS22: lateral ganglionic eminence	Strong	Regionally restricted	3D	Expression is in the ventricular zone of the basal ganglia anlagen (lateral and medial ganglionic eminences in radially aligned clusters of cells.
TS22: medial ganglionic eminence	Strong	Regionally restricted	3D	Expression is in the ventricular zone of the basal ganglia anlagen (lateral and medial ganglionic eminences in radially aligned clusters of cells.
TS22: telencephalon	Strong	Regionally restricted	3D	Expression is in the ventricular zone of the basal ganglia anlagen (lateral and medial ganglionic eminences in radially aligned clusters of cells.

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Present	Regionally restricted	3G	Expression is in the ventricular proliferative zone at the level of the lateral ganglionic eminence, in radially arranged cell clusters.
TS22: telencephalon	Present	Regionally restricted	3G	Expression is in the ventricular proliferative zone at the level of the lateral ganglionic eminence, in radially arranged cell clusters.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	3H	Expression is dorsally in the tectum and in postmitotic neurons of the ventral midbrain.
TS22: hypothalamus	Present	Regionally restricted	3H	Expression is in some nuclei of the developing hypothalamus.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted	3I	Expression is in many populations of differentiating neurons at the midbrain/hindbrain junction.
TS22: midbrain	Present	Regionally restricted	3I	Expression is in many populations of differentiating neurons at the midbrain/hindbrain junction.
TS22: pons	Present	Regionally restricted	3I	Expression is in many populations of differentiating neurons.
TS22: cerebellum	Present		3I

Structure	Level	Pattern	Image	Note
TS22: basal columns	Present	Regionally restricted	3J	Expression is in primary sensory neurons of the developing dorsal horns, and in neurons of the motor pools located ventrally.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		3O
TS28: hippocampus	Present		3O

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Strong		3O inset
TS28: cerebellum Purkinje cell layer	Absent		3O inset