Probe:	mdkk-1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: arterial system	Strong	Regionally restricted	4B	Expression is present in the aortic sac.
TS15: 1st branchial arch mesenchyme	Strong		4B
TS15: septum transversum	Present		4B

Structure	Level	Pattern	Image	Note
TS20: endocardial cushion	Strong		4G	Expression is found in the cushions that develop in the atrioventricular and truncoconal region.
TS20: heart	Strong		4G	Expression is strong in the aortic valve area.
TS20: left atrium	Present	Regionally restricted	4G	Expression is present in the ventral walls.
TS20: right atrium	Present	Regionally restricted	4G	Expression is present in the ventral walls.
TS20: blood vessel	Present		4G	Expression is present in emerging blood vessels.
TS20: pulmonary artery	Weak		4G

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Present		5I	Expression is present in mesoderm, either lateral or superficial.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present	Regionally restricted	5L	Expression is present in the mesenchyme; it is absent from medial regions of developing bone.

Structure	Level	Pattern	Image	Note
TS20: limb pre-cartilage condensation	Present	Regionally restricted	5O	Expression lines the perichondrium; it is not present in medial regions of developing bone.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	6B	Expression is present in mesenchyme condensing around developing whisker and hair follicles, as well as in mesenchyme condensations of the cranium, nasal cartilage and facial ganglia.
TS20: primary palate	Strong	Regionally restricted	6B	Expression is present in the mesoderm immediately adjacent to the surface epithelium.
TS20: vibrissa	Present		6B	Once a hair follicle has formed a vesicle, transcripts spread into the epithelial follicular cells.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	6F	Expression is present in craniofacial mesenchyme adjacent to the vomeronasal organ, as well as in mesenchyme condensations of the cranium, nasal cartilage and facial ganglia.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	8A	Expression is present in a small region of the ventral diencephalon.
TS15: telencephalon	Present	Regionally restricted	8A	Expression is present in a small region.

Structure	Level	Pattern	Image	Note
TS21: eyelid	Present	Regionally restricted	8D	Expression is present in the tips of the developing eyelid.
TS21: pigmented retinal epithelium	Present		8D

Structure	Level	Pattern	Image	Note
TS14: heart	Present			Expression is present in endocardial cells.
TS14: arterial system	Strong	Regionally restricted		Expression is present in the aortic sac.
TS14: 1st branchial arch	Strong

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Strong
TS20: telencephalon mantle layer	Present	Single cells
TS20: spinal cord	Weak
TS20: skeleton	Present	Regionally restricted		Expression is present in the perichondrium of trunk bones.
TS20: eye	Present	Regionally restricted		Expression is present in the tips of the growing eyelids.
TS20: tongue	Present

Structure	Level	Pattern	Image	Note
TS23: limb mesenchyme	Present	Regionally restricted		Expression is present in the mesenchyme surrounding the digits.
TS23: skeleton	Very strong	Regionally restricted		Expression is present in a few cells in mineralising bone, possibly in invading osteoblasts and in epiphyseal cells.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted		Expression is present in the medial mesoderm of the maternal decidua.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Very strong	Regionally restricted		Strong expression is present in the ventral diencephalon.

Structure	Level	Pattern	Image	Note
TS16: bulbus cordis	Present
TS16: septum transversum	Present
TS16: endocardial lining	Present			Expression is present in the endocardium.