Probe:	IMAGE clone 2936937
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS12: cardiogenic plate	Present		2A	Expression is present in the cardiogenic mesoderm and in the forming tubular heart.
TS12: primitive heart tube	Present		2A	Expression is present in the cardiogenic mesoderm and in the forming tubular heart.

Structure	Level	Pattern	Image	Note
TS12: cardiogenic plate	Present		2B	Expression is present in the cardiogenic mesoderm and in the forming tubular heart.
TS12: primitive heart tube	Present		2B	Expression is present in the cardiogenic mesoderm and in the forming tubular heart.
TS12: pre-otic sulcus	Weak	Regionally restricted	2B	Expression is present in the lateral neural folds in the region of the pre-otic sulcus.

Structure	Level	Pattern	Image	Note
TS12: heart	Present		2C	Expression is present in the tubular heart.

Structure	Level	Pattern	Image	Note
TS12: heart	Strong	Uniform	2D	Expression is present in the tubular heart.
TS12: neural ectoderm	Present	Regionally restricted	2D	Expression in the neural folds extends anteriorly towards the isthmus and posteriorly into the caudal neural tube.

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Present	Regionally restricted	2E	Expression in the neural folds extends anteriorly towards the isthmus and posteriorly into the caudal neural tube.
TS14: neural tube floor plate	Present		2E

Structure	Level	Pattern	Image	Note
TS15: heart atrium	Present		2F	Expression is stronger in the ventricle as compared to the atria.
TS15: primitive ventricle	Present		2F	Expression is stronger in the ventricle as compared to the atria.
TS15: dorsal root ganglion	Present	Regionally restricted	2F	Expression is present in the anteriormost dorsal root ganglia.
TS15: central nervous system	Present	Regionally restricted	2F	Expression is restricted to the dorsal neural tube adjacent to the roof plate.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	2G	Expression is restricted to the dorsal neural tube adjacent to the roof plate.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Regionally restricted	2H	Expression is present in the dorsomedial telencephalon.
TS15: cranial preganglion	Present		2H
TS15: dorsal root ganglion	Present	Regionally restricted	2H	Expression is present in the first forming dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Present		2I
TS17: eye	Present	Regionally restricted	2I	Expression is present in the dorsal eye.
TS17: central nervous system	Present	Regionally restricted	2I	Expression is present in the neural folds.
TS17: telencephalon	Present	Regionally restricted	2I	Expression is present in the dorsomedial telencephalon.
TS17: dorsal root ganglion	Present		2I
TS17: heart	Present		2I

Structure	Level	Pattern	Image	Note
TS19: nasal pit	Present		2J	Expression is present in the nasal placode.
TS19: central nervous system	Present	Regionally restricted	2J	Expression is present in the neural folds.
TS19: telencephalon	Present	Regionally restricted	2J	Expression is present in the dorsomedial telecephalon.
TS19: cranial ganglion	Present		2J
TS19: dorsal root ganglion	Present		2J
TS19: heart	Present		2J

Structure	Level	Pattern	Image	Note
TS17: rhombomere 4	Weak	Regionally restricted	3A	Expression is present in rhombomere 4 facial branchiomotor neurons.
TS17: hindbrain	Present	Regionally restricted	3A	Expression is present in the dorsal hindbrain.

Structure	Level	Pattern	Image	Note
TS19: trigeminal ganglion	Present		3C	Expression is present in rhombomere 3 trigeminal motor neurons.
TS19: hindbrain	Present	Regionally restricted	3C	Expression is present in facial branchiomotor neurons migrating caudally and then laterally into rhombomere 6.

Structure	Level	Pattern	Image	Note
TS19: cranial nerve	Present	Regionally restricted	3E	Expression is present in the oculomotor nucleus.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3G	Expression is present in the dorsal hindbrain. It is also is present in facial branchiomotor neurons migrating caudally and then laterally into rhombomere 6.
TS20: embryo	Present	Regionally restricted	3G	Expression is present in a dorsal longitudinal column of cells.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	4A	Expression is present in the dorsomedial telencephalon.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4C	Expression is present in the dorsomedial telencephalon.
TS19: diencephalon	Present	Regionally restricted	4C	Expression is present in the dorsal diencephalon.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4E	Expression is present in the dorsomedial telencephalon.
TS20: diencephalon	Present	Regionally restricted	4E	Expression is present in the dorsal diencephalon.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4G	Expression extends ventrally and dorsally in a gradient, including into the anlagen of the hippocampus. and the cortical hem, a signalling center which directs formation of the hippocampus.
TS20: choroid invagination	Present		4G	Expression is present in the anlagen of the choroid plexus.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	4I	Expression is present in the dorsomedial telencephalon.
TS19: diencephalon	Present	Regionally restricted	4I	Expression is present in the hypothalamus.
TS19: cranial nerve	Present	Regionally restricted	4I	Expression is present in the oculomotor nucleus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4J	Expression is present in the dorsomedial and ventral telencephalon.
TS20: hypothalamus	Present		4J
TS20: oculomotor nerve	Present		4J