Probe:	PBC/5-276S
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors state that probe PBC/5-276S wa used for Fig. 3A and that almost the same signals were detected using probe PBC/BS or PBC/PX (MGI:3588138 or MGI:3588139 respectively). It was not indicated which probe was used with subsequent specimens.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present		3A right embryo
TS17: forelimb bud	Present		3A right embryo
TS17: hindlimb bud	Present		3A right embryo
TS17: midbrain	Strong		3A right embryo
TS17: telencephalon	Strong		3A right embryo
TS17: hindbrain	Strong	Graded	3A right embryo	Expression is stronger in the myelencephalon.
TS17: future spinal cord	Present	Graded	3A right embryo	Expression decreased along the anterior-posterior axis with trace amounts at the lumbar and sacral level.
TS17: branchial arch	Absent		3A right embryo
TS17: gut	Absent		3A right embryo
TS17: tail somite	Absent		3A right embryo
TS17: trunk somite	Absent		3A right embryo

Structure	Level	Pattern	Image	Note
TS17: midbrain	Strong		3B
TS17: telencephalon	Strong		3B
TS17: hindbrain	Strong	Graded	3B	Expression is stronger in the myelencephalon.
TS17: diencephalon	Present		3B
TS17: future spinal cord	Present	Graded	3B	Expression decreased along the anterior-posterior axis, with trace amounts at the lumbar and sacral level.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	3C	Expression is in the ventrolateral horns, and is weak ventromedially.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Strong	Regionally restricted	4A	Expression is in a sharp, narrow band of cells that extended from the dorsal midline around the lateral walls of the neural tube just anterior of the isthmus lying at the mesencephalon/metencephalon junction. Weak expression in the mesencephalon.
TS17: midbrain	Strong	Regionally restricted	4A	Expression is in a sharp, narrow band of cells that extended from the dorsal midline around the lateral walls of the neural tube just anterior of the isthmus lying at the mesencephalon/metencephalon junction. Weak expression in the mesencephalon.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	5A	Expression is in the mesodermal cells at the posterior region of the bud.

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Strong	Regionally restricted	5C	Expression is in a distal region spreading anteriorly and a proximal, posteriorly limited region.

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Present	Regionally restricted		Expression is absent the distal region and in the proximal, posteriorly limited region.

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit	Present	Regionally restricted	5D	Expression was localized in a domain around the digit rudiments.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present
TS22: olfactory lobe	Present