Probe:	Daam1 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Weak
TS10: extraembryonic ectoderm	Weak

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		2A
TS11: extraembryonic component	Present		2A

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		2B
TS13: extraembryonic component	Present		2B
TS13: yolk sac mesenchyme	Strong		2B
TS13: head somite	Present		2B
TS13: trunk somite	Present		2B

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present		2C
TS15: diencephalon	Present		2C
TS15: future hindbrain	Present		2C
TS15: lens placode	Present		2C
TS15: optic cup intraretinal space	Present		2C
TS15: optic vesicle	Present		2C
TS15: facio-acoustic preganglion complex	Present		2C
TS15: glossopharyngeal preganglion	Present		2C
TS15: trigeminal preganglion	Present		2C
TS15: dorsal root ganglion	Strong		2C
TS15: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present		2C
TS15: 1st branchial arch maxillary component mesenchyme derived from neural crest	Present		2C
TS15: 1st branchial pouch	Present		2C
TS15: 2nd branchial arch mesenchyme derived from neural crest	Present		2C
TS15: 2nd branchial pouch	Present		2C
TS15: 3rd branchial arch mesenchyme derived from neural crest	Present		2C
TS15: 3rd branchial pouch	Present		2C
TS15: forelimb bud mesenchyme	Weak	Uniform	2C
TS15: hindlimb ridge mesenchyme	Weak	Uniform	2C

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present		2D
TS17: trunk somite	Present		2D
TS17: diencephalon	Present		2D
TS17: hindbrain	Present		2D
TS17: lens pit	Present	Regionally restricted	2D
TS17: optic cup	Present	Regionally restricted	2D
TS17: acoustic ganglion	Present		2D
TS17: facial ganglion	Present		2D
TS17: glossopharyngeal ganglion	Present		2D
TS17: trigeminal ganglion	Present		2D
TS17: dorsal root ganglion	Strong		2D
TS17: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present		2D
TS17: 1st branchial arch maxillary component mesenchyme derived from neural crest	Present		2D
TS17: 1st branchial pouch	Present		2D
TS17: 2nd branchial arch mesenchyme derived from neural crest	Present		2D
TS17: 2nd branchial pouch	Present		2D
TS17: 3rd branchial arch mesenchyme derived from neural crest	Present		2D
TS17: 3rd branchial pouch	Present		2D
TS17: limb mesenchyme	Weak	Uniform	2D

Structure	Level	Pattern	Image	Note
TS17: tail somite	Present		2E
TS17: trunk somite	Present		2E
TS17: diencephalon	Present		2E
TS17: hindbrain	Present		2E
TS17: future spinal cord	Present	Regionally restricted	2E	Ventrolateral expression.
TS17: midgut	Present	Regionally restricted	2E	Expression is in the mesenchyme.
TS17: dorsal root ganglion	Strong		2E
TS17: latero-nasal process	Absent		2E
TS17: medial-nasal process	Absent		2E

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present	Regionally restricted	2F	Expression is in posterior vertebrae.
TS20: vertebral cartilage condensation	Present	Regionally restricted	2F	Expression is in posterior vertebrae.
TS20: forebrain	Strong		2F
TS20: hindbrain	Strong		2F
TS20: midbrain	Strong		2F
TS20: spinal cord	Strong	Regionally restricted	2F	Ventrolateral expression.
TS20: lens vesicle	Strong		2F
TS20: retina	Strong		2F
TS20: vibrissa	Weak		2F
TS20: gut	Present		2F
TS20: hindgut mesenchyme	Present		2F
TS20: midgut	Present		2F
TS20: intestine	Present		2F
TS20: hand	Present	Regionally restricted	2F	Expression is in the distal-most limb mesenchyme and the metacarpal/phalange joints.
TS20: forelimb interdigital region	Weak		2F

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		4A

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	4C	Ventrolateral expression.

Structure	Level	Pattern	Image	Note
TS17: lung mesenchyme	Present	Single cells	4E
TS17: lung epithelium	Strong		4E

Structure	Level	Pattern	Image	Note
TS17: stomach epithelium	Present		4G
TS17: stomach mesenchyme	Present	Regionally restricted	4G	Expression is in the posterior mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: liver	Strong	Single cells	4I

Structure	Level	Pattern	Image	Note
TS22: forebrain	Strong
TS22: hindbrain	Strong
TS22: midbrain	Strong
TS22: spinal cord	Strong	Regionally restricted		Ventrolateral expression.
TS22: intestine	Present	Regionally restricted		Expression is in the ceca.
TS22: vibrissa	Absent

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	5A	Expression is in the distal-most limb mesenchyme and the metacarpal/phalange joints.
TS20: hand	Present	Regionally restricted	5A	Expression is in the distal-most limb mesenchyme and the metacarpal/phalange joints.
TS20: forelimb interdigital region	Weak		5A

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	5B	Expression is in the joints and perichondrial regions.