Probe:	Msg1 cDNA2
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS10: primitive endoderm	Present	Regionally restricted	3A	Expression in the proximal embryonic visceral endoderm was asymmetrical, extending slightly further distally on the posterior side of the egg cylinder.
TS10: extraembryonic visceral endoderm	Present		3A
TS10: extraembryonic ectoderm	Present		3A

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	3B	Expression is in nascent mesoderm.
TS10: primitive streak	Present	Regionally restricted	3B	Expression is in the most posterior part.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic visceral endoderm	Present		3C
TS11: embryo mesoderm	Present	Regionally restricted	3C	Expression is in the mesoderm adjacent to the primitive streak.
TS11: chorion ectoderm	Present		3C
TS11: yolk sac endoderm	Present		3C

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	3D	Expression is in the mesodermal component.
TS11: embryo mesoderm	Present	Regionally restricted	3D	Expression is in the mesoderm adjacent to the primitive streak and is absent in the more mature mesoderm underlying the neural folds.
TS11: extraembryonic visceral endoderm	Present		3D

Structure	Level	Pattern	Image	Note
TS12: cardiogenic plate	Present		4A
TS12: yolk sac endoderm	Present		4A

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present	Regionally restricted	4B	Expression is in the posterior of the fusing heart tube.

Structure	Level	Pattern	Image	Note
TS12: heart	Present	Regionally restricted	4C	Expression is in a posteroventral domain of the developing heart.

Structure	Level	Pattern	Image	Note
TS13: primitive ventricle	Present		4D

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Present		4E
TS14: primitive streak	Present		4E
TS14: primitive ventricle	Present		4E
TS14: trunk unsegmented mesenchyme	Present		4E
TS14: future hindbrain	Present	Regionally restricted	4E	Expression is in the neural crest.
TS14: future midbrain	Present	Regionally restricted	4E	Expression is in the neural crest.
TS14: prosencephalon	Present	Regionally restricted	4E	Expression is in the neural crest.

Structure	Level	Pattern	Image	Note
TS12: yolk sac endoderm	Present		4F
TS12: cardiac muscle tissue	Present	Regionally restricted	4F	Expression is in the ventral myocardium.
TS12: endocardial tube	Absent		4F	Absent in the endocardium.
TS12: gut endoderm	Absent		4F

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle	Present		4G	Expression is in the left ventricle and future right ventricle.
TS15: heart atrium	Weak		4G

Structure	Level	Pattern	Image	Note
TS17: primitive ventricle cardiac muscle	Present		4H
TS17: primitive ventricle	Present	Single cells	4H	Expression is in the trabeculae.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present	Regionally restricted	5A	Expression is in lateral mesoderm adjacent to somites 6-10.
TS13: trunk somite	Present	Regionally restricted	5A	Expresion is in nascent somites, in more mature somites the expression is in the posterior aspect of the sclerotome.
TS13: head somite	Present	Regionally restricted	5A	Expression is in the posterior aspect of the sclerotome.
TS13: primitive streak	Present		5A
TS13: trunk unsegmented mesenchyme	Present		5A

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	5B	Expression is in the primitive streak.
TS15: forelimb bud mesenchyme	Present		5B
TS15: tail unsegmented mesenchyme	Present		5B
TS15: trunk somite	Present	Regionally restricted	5B	Expresion is in nascent somites.
TS15: trunk sclerotome	Present	Regionally restricted	5B	In more mature somites the expression is posterior.
TS15: 1st branchial arch	Present		5B
TS15: head mesenchyme derived from neural crest	Present		5B

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5D , 5C	Expression is in the posteriodorsal mesenchyme.
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	5C , 5D	Expression is in the posterior mesenchyme.
TS17: tail	Present		5C , 5D
TS17: tail unsegmented mesenchyme	Present		5C , 5D
TS17: tail sclerotome	Present	Regionally restricted	5D , 5C	Expression is in the posterior sclerotome.
TS17: trunk sclerotome	Present	Regionally restricted	5D , 5C	Expression is in the posterior sclerotome.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	5E	Expression is in the dorsal mesenchyme, and is absent in the most posterior aspect and the distal tip.
TS20: hindlimb	Present	Graded	5E	Expression is strong dorsally and weak ventrally.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present	Graded	5F	Expression is in the dorsal mesenchyme and is weak in the ventral mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: trunk sclerotome	Present	Regionally restricted	5G	Expression is in the posterior sclerotome of mature somites.

Structure	Level	Pattern	Image	Note
TS17: nephric duct	Present		5H

Structure	Level	Pattern	Image	Note
TS21: heart	Present
TS21: tail	Present
TS21: head	Present	Regionally restricted		Expression is in the branchial arches.
TS21: trunk	Absent			Absent in the somite derivatives in the trunk.
TS21: liver	Absent