Probe:	pEL107
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Another probe, PEL111 (MGI:9294) produced similar results.

Structure	Level	Pattern	Image	Note
TS13: yolk sac	Present

Structure	Level	Pattern	Image	Note
TS15: trunk sclerotome	Present	Regionally restricted	4A	Expression was detected in the sclerotime of differentiating somites.
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	4A	Expression was detected in the sclerotome but was absent from the dermamyotome, the newly segmented somites and the presomitic mesoderm.
TS15: trunk dermomyotome	Absent		4A
TS15: notochord	Present		4A

Structure	Level	Pattern	Image	Note
TS20: trunk mesenchyme	Present	Regionally restricted	4B	Expression was detected in the perinotochordal condensation of sclerotome in the caudal region.

Structure	Level	Pattern	Image	Note
TS20: axial skeleton	Present	Regionally restricted	4C	Expression was detected in the prevertebrae in the upper trunk region.
TS20: skin	Present	Regionally restricted	4C	Expression was detected in the surface ectoderm.
TS20: spinal ganglion	Absent		4C

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	4D	Expression was detected in the cranial mesenchyme adjacent to the hypophyseal pouch.
TS17: embryo mesenchyme	Present	Regionally restricted	4D	Expression was detected in the ectomesenchyme beneath the surface ectoderm.
TS17: otocyst	Present		4D

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Present	Regionally restricted	4E	Expression was detected in chondrocytes in the developing centrum of the vertebrae.
TS20: skin	Present	Regionally restricted	4E	Expression was detected in the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present	Regionally restricted		Expression was detected in the cranial mesenchyme associated with the mid- and hindbrain.

Structure	Level	Pattern	Image	Note
TS20: embryo mesenchyme	Present	Regionally restricted	4F	Expression was detected in mesenchymal cell masses destined to be the hyoid bone and the thyroid cartilage.
TS20: inner ear mesenchyme	Present	Regionally restricted	4F	Expression was detected in the periotic mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: chondrocranium	Present		4G
TS20: head mesenchyme	Present	Regionally restricted	4G	Expression was detected in the superficial mesenchyme over the cerebral vesicles destined for the frontal bone and part of the parietal bone and that of the supraoccipital bone.
TS20: forebrain	Present	Regionally restricted	4G	Expression was detected in cells in the hippocampal cortex.
TS20: cerebral cortex ventricular layer	Present		4G
TS20: diencephalon	Present		4G
TS20: midbrain	Present		4G
TS20: 4th ventricle	Present	Regionally restricted	4G	Expression was detected in the floor of the 4th ventricle in the rhombencephalon.
TS20: embryo mesenchyme	Present	Regionally restricted	4G	Expression was detected in the ectomesenchyme beneath the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS22: vault of skull	Present	Regionally restricted	4H	Expression was detected in the calvaria.
TS22: epidermis	Present	Regionally restricted	4H	Expression was detected in differentiating epidermis.
TS22: axial skeleton	Present		4H
TS22: chondrocranium	Present		4H
TS22: jaw	Present		4H
TS22: limb cartilage condensation	Present		4H
TS22: nasal septum	Present		4H
TS22: otic capsule	Present		4H
TS22: rib cartilage condensation	Present		4H
TS22: trachea	Present	Regionally restricted	4H	Expression was detected in the tracheal rings.
TS22: embryo mesenchyme	Present	Regionally restricted	4H	Expression was detected in the ectomesenchyme beneath the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present		5A
TS17: trunk sclerotome	Present		5A
TS17: notochord	Strong		5A

Structure	Level	Pattern	Image	Note
TS15: notochord	Strong
TS15: cardiac muscle tissue	Present
TS15: visceral pericardium	Present			Expression was detected in the epicardium.
TS15: endocardial lining	Absent
TS15: parietal pericardium	Absent			Expression was absent from the pericardium.

Structure	Level	Pattern	Image	Note
TS20: notochord	Strong
TS20: cardiac muscle tissue	Present
TS20: visceral pericardium	Present			Expression was detected in the epicardium.
TS20: endocardial lining	Absent
TS20: parietal pericardium	Absent			Expression was absent from the pericardium.

Structure	Level	Pattern	Image	Note
TS17: common atrial chamber cardiac muscle	Present		5B
TS17: primitive ventricle cardiac muscle	Present		5B
TS17: visceral pericardium	Present		5B	Expression was detected in the epicardium.
TS17: endocardial lining	Absent		5B
TS17: parietal pericardium	Absent		5B	Expression was absent from the pericardium.

Structure	Level	Pattern	Image	Note
TS24: tail	Present	Regionally restricted	5C	Expression was detected in the tail tendon.

Structure	Level	Pattern	Image	Note
TS26: tail	Present	Regionally restricted		Expression was detected in the tail tendon.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Weak	Regionally restricted		Expression was detected in the neuroepithelium.
TS15: otocyst epithelium	Present

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted		Expression was detected in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS21: diencephalon ventricular layer	Present		5D
TS21: midbrain floor plate	Present		5D
TS21: diencephalon	Present	Regionally restricted	5D	Expression was detected in the vesicle floor.
TS21: diencephalon mantle layer	Weak		5D

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present		5E
TS22: diencephalon	Present		5E
TS22: thalamus	Present	Regionally restricted	5E	Expression was detected in the neuroblasts of the thalamic nuclei.

Structure	Level	Pattern	Image	Note
TS22: cornea epithelium	Present		5F
TS22: neural retinal epithelium	Present		5F
TS22: conjunctival sac	Present	Regionally restricted	5F	Expression was detected in the conjunctival epithelium.
TS22: perioptic mesenchyme	Present	Regionally restricted	5F	Expression was detected in the sclera.
TS22: lens	Absent		5F
TS22: pigmented retinal epithelium	Absent		5F
TS22: eyelid	Weak	Regionally restricted	5F	Expression was weak in the mesenchyme of the eyelids.

Structure	Level	Pattern	Image	Note
TS22: hindbrain basal plate	Present	Regionally restricted	5G	Expression was detected in the neuroepithelial cells in the basal plate of the upper rhombencephalon.
TS22: head mesenchyme	Strong	Regionally restricted	5G	Expression was detected in the mesenchyme of the prospective occipital bone.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata basal plate ventricular layer	Present		5H
TS22: spinal cord ventricular layer	Present	Regionally restricted	5H	Expression was detected in the ventricular cell layer on the basal side of the cervical spinal cord.
TS22: axial skeleton cervical region	Strong		5H
TS22: spinal cord	Present	Regionally restricted	5H	Expression was detected in the ventricular cell layer but was absent from mature tissue of the spinal cord.
TS22: spinal ganglion	Absent		5H

Structure	Level	Pattern	Image	Note
TS24: brain	Weak	Regionally restricted		Expression was detected in the ventricular and intermediate cell layers.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	5I	Expression was concentrated at the apical cytoplasm.
TS17: otocyst mesenchyme	Present		5I

Structure	Level	Pattern	Image	Note
TS22: inner ear mesenchyme	Present	Regionally restricted	5J	Expression was detected in the periotic mesenchyme.
TS22: cochlear duct epithelium	Present		5J
TS22: otic capsule	Present		5J