Probe:	Crim1 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS14: trunk somite	Present	Regionally restricted	3A	Expression is in the central portion of rostrally located somites of the body.

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Present	Regionally restricted	3B	Expression is in the ventral region.
TS16: future midbrain	Present	Regionally restricted	3B	Expression is in the ventral region.

Structure	Level	Pattern	Image	Note
TS18: trunk somite	Present		3C	Expression is in more caudal somites.
TS18: lens vesicle	Present		3C
TS18: neural tube	Present	Regionally restricted	3C	Expression is in the ventral aspect.
TS18: 1st branchial arch mandibular component	Present	Regionally restricted	3C	Expression is in a portion of the developing tooth primordia.
TS18: 1st branchial arch maxillary component	Present	Regionally restricted	3C	Expression is in a portion of the developing tooth primordia.
TS18: otocyst	Present	Regionally restricted	3C	Expression is in a portion of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS21: lens	Present		3D
TS21: pinna	Present		3D
TS21: tooth	Present		3D
TS21: vibrissa	Present		3D
TS21: nose	Present	Regionally restricted	3D	Expression is in the nasal epithelium.

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present		3E
TS19: neural tube	Present	Regionally restricted	3E
TS19: mandibular process	Present	Regionally restricted	3E	Expression is in a portion of the developing tooth primordia.
TS19: maxillary process	Present	Regionally restricted	3E	Expression is in a portion of the developing tooth primordia.
TS19: inner ear	Present	Regionally restricted	3E	Expression is in a portion of the otic vesicle.
TS19: diencephalon	Present	Regionally restricted	3E	Expression is in the pretectum and zona limitans and in a region posterior to the optic stalk.
TS19: metencephalon roof	Present	Regionally restricted	3E	Expression is in the roof of the anterior hindbrain.
TS19: metencephalon floor plate	Present		3E
TS19: midbrain floor plate	Present		3E
TS19: myelencephalon floor plate	Present		3E

Structure	Level	Pattern	Image	Note
TS21: tooth	Present		3F
TS21: medulla oblongata floor plate	Strong		3F
TS21: metencephalon floor plate	Strong		3F
TS21: midbrain floor plate	Strong		3F
TS21: hindbrain	Strong	Regionally restricted	3F	Expression is in an intermediate region.
TS21: diencephalon	Strong	Regionally restricted	3F	Expression is in the dorsal midline of the rostral end of the diencephalon.

Structure	Level	Pattern	Image	Note
TS21: tooth	Present		3G
TS21: nasal cavity epithelium	Present		3G

Structure	Level	Pattern	Image	Note
TS21: lens	Present		3H

Structure	Level	Pattern	Image	Note
TS21: pinna	Present		3I

Structure	Level	Pattern	Image	Note
TS19: metencephalon floor plate	Present		3J
TS19: myelencephalon floor plate	Present		3J
TS19: trigeminal ganglion	Absent		3J

Structure	Level	Pattern	Image	Note
TS19: trigeminal ganglion	Absent		3K

Structure	Level	Pattern	Image	Note
TS19: metencephalon floor plate	Present		3L
TS19: myelencephalon floor plate	Present		3L

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	4A	Expression is in the floor plate.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	4C	Expression is in the motor neurons in the ventro-lateral part.
TS17: neural tube floor plate	Present		4C
TS17: notochord	Present		4C

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Regionally restricted	4E	Expression is in a subset of motor neurons at the dorsal and medial regions of the motor column.
TS19: neural tube floor plate	Present		4E

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present		4H

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Regionally restricted	4I	Expression is in the medial region ofthe motor column. Section is at the thoracic level.
TS19: neural tube floor plate	Present		4I

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present		4L

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Weak		4M
TS21: spinal cord lateral wall	Weak	Regionally restricted	4M	Expression is in the LMC (lateral motor column) motor neurons, and in few motor neurons in the lateral part of the MMC (medial motor column). Section is at the brachial level.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present		4P

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Weak		4Q
TS21: spinal cord lateral wall	Present	Regionally restricted	4Q	Expression is in an intermediate region of the spinal cord.
TS21: basal columns	Present	Regionally restricted	4Q	Expression is in the medial motor columns and is absent in the preganglionic motor neurons which have migrated to the lateral horn of the thoracic spinal cord.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present		4T

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Strong		4U
TS23: spinal cord	Present		4U

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Strong		4W
TS23: spinal cord	Present		4W