Gene Expression Data
Assay Details
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Assay
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Reference:
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J:47699
Pabst O, et al., Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Mech Dev. 1998 Apr;73(1):85-93
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3029967
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Gene symbol:
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Nkx2-9
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Gene name:
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NK2 homeobox 9
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Probe:
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Nkx2-9 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The authors state that several probes were used for the RNA in situ analysis which include these two smaller probes: Nkx2-9 cDNA3 (MGI:1270229), and Nkx2-9 cDNA4 (MGI:1270230). All probes gave similar results with some variations in signal intensities.
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Results
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Specimen
3A:
embryonic day 7.0
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 7.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo endoderm |
Present |
Regionally restricted |
3A
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Expression is in the anterior part of the endoderm underlying the neural plate.
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Specimen
3B:
embryonic day 7.0
(more )
Specimen
3B:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 7.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo endoderm |
Present |
Regionally restricted |
3B
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Expression is in the anterior part of the endoderm underlying the neural plate.
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Specimen
3C:
embryonic day 7.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo endoderm |
Present |
Regionally restricted |
3C
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Expression is in the anterior part of the endoderm underlying the neural plate and has expanded caudally in its medial portion.
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| TS11: embryo mesoderm |
Present |
Regionally restricted |
3C
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Expression is in two bilateral stripes.
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Specimen
3D:
embryonic day 7.5
(more )
Specimen
3D:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo endoderm |
Present |
Regionally restricted |
3D
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Expression is in the anterior part of the endoderm underlying the neural plate and has expanded caudally in its medial portion.
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| TS11: embryo ectoderm |
Present |
Regionally restricted |
3D
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Expression is in the ventral tip of the neural tube.
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| TS11: embryo mesoderm |
Present |
Regionally restricted |
3D
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Expression is in two bilateral stripes.
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Specimen
3E:
embryonic day 8.5
(more )
Note: Up to 10 somites.
Specimen
3E:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Up to 10 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
3E
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Expression is in small domains.
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| TS12: neural tube |
Present |
Regionally restricted |
3E
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Expression is in small domains.
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Specimen
3F:
embryonic day 8.5
(more )
Note: Up to 10 somites.
Specimen
3F:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Up to 10 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Regionally restricted |
3F
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Expression is ventral and in small domains.
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| TS13: neural tube |
Present |
Regionally restricted |
3F
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Expression is ventral and in small domains.
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Specimen
3G:
embryonic day 8.5
(more )
Note: Up to 10 somites.
Specimen
3G:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Up to 10 somites.
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Specimen
3H:
embryonic day 9.5
(more )
Specimen
3H:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3I:
embryonic day 9.5
(more )
Specimen
3I:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future brain |
Present |
Regionally restricted |
3I
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Expression is in two parallel stripes along the brain, at the anterior and posterior end the stripes fuse to a 'V'-shape.
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Specimen
3J:
embryonic day 9.5
(more )
Specimen
3J:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future brain |
Present |
Regionally restricted |
3J
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Expression is in two parallel stripes along the brain, at the anterior and posterior end the stripes fuse to a 'V'-shape.
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Specimen
3K:
embryonic day 9.5
(more )
Specimen
3K:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
3L:
embryonic day 10.5
(more )
Specimen
3L:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain |
Strong |
Regionally restricted |
3L
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Expression is in dorsal domains.
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| TS17: neural tube |
Present |
Regionally restricted |
3L
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Expression is in the caudal part of the neural tube.
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Specimen
3M:
embryonic day 10.5
(more )
Specimen
3M:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
3N:
embryonic day 12.5
(more )
Specimen
3N:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: brain |
Present |
Regionally restricted |
3N
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Expression is in the ventricular zone.
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Specimen
3O:
embryonic day 12.5
(more )
Specimen
3O:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Specimen
4B:
embryonic day 8.0
(more )
Note: 3 somites.
Specimen
4B:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
4B
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Expression is in a 'V'-shaped structure at the anterior aspect of the brain.
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Specimen
4D:
embryonic day 8.0
(more )
Note: 8 somites.
Specimen
4D:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
4D
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Expression is in two parallel stripes along the brain, at the anterior and posterior end the stripes fuse to a 'V'-shape.
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Specimen
4E:
embryonic day 8.0
(more )
Note: 6 somites. Double labeled: red - Nkx2-9; blue - Nkx2-2 (assay MGI:3032438).
Specimen
4E:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites. Double labeled: red - Nkx2-9; blue - Nkx2-2 (assay MGI:3032438).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future prosencephalon |
Present |
Regionally restricted |
4E
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The expression domain overlapsoverlaps with Nkx2-2.
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Specimen
4F:
embryonic day 8.0
(more )
Note: 6 somites. Double labeled: red - Nkx2-9; blue - Nkx2-2 (assay MGI:3032438).
Specimen
4F:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites. Double labeled: red - Nkx2-9; blue - Nkx2-2 (assay MGI:3032438).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future prosencephalon |
Present |
Regionally restricted |
4F
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The expression domain overlapsoverlaps with Nkx2-2.
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Specimen
4G:
embryonic day 8.0
(more )
Note: 6 somites. Double labeled: red - Nkx2-9; blue - Nkx2-2 (assay MGI:3032438).
Specimen
4G:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somites. Double labeled: red - Nkx2-9; blue - Nkx2-2 (assay MGI:3032438).
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Specimen
4H:
embryonic day 8.0
(more )
Note: 4 somites. Double labeled: blue - Nkx2-9; red - Shh (assay MGI:3032440).
Specimen
4H:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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4 somites. Double labeled: blue - Nkx2-9; red - Shh (assay MGI:3032440).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
Regionally restricted |
4H
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Expression is in a domain directly adjacent to the expression domain of Shh.
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| TS12: future prosencephalon |
Present |
Regionally restricted |
4H
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Expression is in a domain directly adjacent to the expression domain of Shh.
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Specimen
4I:
embryonic day 8.0
(more )
Note: 4 somites. Double labeled: blue - Nkx2-9; red - Shh (assay MGI:3032440).
Specimen
4I:
(close )
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Genetic Background:
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ICR
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Age:
|
embryonic day 8.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Note:
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4 somites. Double labeled: blue - Nkx2-9; red - Shh (assay MGI:3032440).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube |
Present |
Regionally restricted |
4I
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Expression is in a domain directly adjacent to the expression domain of Shh.
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Specimen
4J:
embryonic day 8.5
(more )
Note: 10 somites. Double labeled: blue - Nkx2-9; red - Shh (assay MGI:3032440).
Specimen
4J:
(close )
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Genetic Background:
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ICR
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Age:
|
embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
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Type:
|
section
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|
Fixation:
|
4% Paraformaldehyde
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|
Note:
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10 somites. Double labeled: blue - Nkx2-9; red - Shh (assay MGI:3032440).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
Regionally restricted |
4J
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Expression is in a domain directly adjacent to the expression domain of Shh.
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| TS13: future prosencephalon |
Present |
Regionally restricted |
4J
|
Expression is in a domain directly adjacent to the expression domain of Shh.
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Specimen
4K:
embryonic day 8.5
(more )
Note: 10 somites. Double labeled: blue - Nkx2-9; red - Shh (assay MGI:3032440).
Specimen
4K:
(close )
|
Genetic Background:
|
ICR
|
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Age:
|
embryonic day 8.5
|
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
10 somites. Double labeled: blue - Nkx2-9; red - Shh (assay MGI:3032440).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural tube |
Present |
Regionally restricted |
4K
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Expression is in a domain directly adjacent to the expression domain of Shh.
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Specimen
Not Shown:
embryonic day 13.5
(more )
Specimen
Not Shown:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 13.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
|
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Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: diencephalon |
Present |
Regionally restricted |
|
Expression is in a small region of the ventral diencephalon.
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Specimen
Not Shown:
embryonic day 14.5
(more )
Specimen
Not Shown:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.5
|
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
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Sex:
|
Not Specified
|
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Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
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Analysis Tools