Gene Expression Data
Assay Details
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Assay
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Reference:
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J:87380
Camurri L, et al., Rig-1 a new member of Robo family genes exhibits distinct pattern of expression during mouse development. Gene Expr Patterns. 2004 Jan;4(1):99-103
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3026881
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Gene symbol:
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Robo3
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Gene name:
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roundabout guidance receptor 3
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Probe:
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Rig-1 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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Some sectioned embryos were embedded in 30% gelatin, fixed in 4% formaldehyde and sectioned at 50 microns with a vibratome. Some embryos were embedded in gelatin/albumin/sucrose/ and fixed with 2.5% gluteraldehyde and sectioned at 80 microns. The authorsdid not specify for each section which method was used.
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Results
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Specimen
1A:
embryonic day 8.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future prosencephalon |
Present |
Regionally restricted |
1A
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Expression is in the ventral part of the forebrain.
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Specimen
1B:
embryonic day 8.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future prosencephalon |
Present |
Regionally restricted |
1B
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Expression is in the ventral part of the forebrain.
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Specimen
1C:
embryonic day 9.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1D:
embryonic day 9.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1E:
embryonic day 10.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain roof plate |
Present |
Regionally restricted |
1E
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Expression is in the developing tectum on either side of the midline starting at the the fore- and midbrain boundary with a gap at the caudal midbrain.
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| TS17: hindbrain |
Present |
Regionally restricted |
1E
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Expression is in the upper rhombic lip to the caudal hindbrain where it is weakly expressed.
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| TS17: future spinal cord |
Present |
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1E
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Specimen
1F:
embryonic day 10.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain roof plate |
Present |
Regionally restricted |
1F
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Expression is in the developing tectum on either side of the midline starting at the the fore- and midbrain boundary with a gap at the caudal midbrain.
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| TS17: hindbrain |
Present |
Regionally restricted |
1F
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Expression is in the upper rhombic lip to the caudal hindbrain where it is weakly expressed.
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| TS17: future spinal cord |
Present |
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1F
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Specimen
1G:
embryonic day 11.5
(more )
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1H:
embryonic day 11.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1I:
embryonic day 12.5
(more )
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
1J:
embryonic day 12.5
(more )
Specimen
1J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
2A:
embryonic day 8.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Specimen
2B:
embryonic day 8.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Specimen
2C:
embryonic day 10.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Regionally restricted |
2C
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Expression in the lateral walls of the anterior part of the vesicle.
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Specimen
2D:
embryonic day 10.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Specimen
2E:
embryonic day 11.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain roof plate |
Present |
Regionally restricted |
2E
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Expression is in the rostral and caudal tectum.
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| TS19: telencephalon |
Present |
Regionally restricted |
2E
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Expression is laterally in periventricular neurons in the walls of the telencephalic vesicles.
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Specimen
2F:
embryonic day 11.5
(more )
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Strong |
Regionally restricted |
2F
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Expression is in the dorso-lateral area. Expression in an area corresponding to rhombomre 1.
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Specimen
2G:
embryonic day 11.5
(more )
Specimen
2G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Strong |
Regionally restricted |
2G
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Expression is in the dorso-lateral area.
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Specimen
2H:
embryonic day 11.5
(more )
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain roof plate |
Present |
Regionally restricted |
2H
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Expression is in the rostral and caudal tectum.
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Specimen
2I:
embryonic day 11.5
(more )
Specimen
2I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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|
Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
2I
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Expression is laterally in periventricular neurons in the walls of the telencephalic vesicles.
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Specimen
2J:
embryonic day 11.5
(more )
Specimen
2J:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain roof plate |
Present |
Regionally restricted |
2J
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Expression is in the rostral and caudal tectum.
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Specimen
2K:
embryonic day 11.5
(more )
Specimen
2K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: midbrain roof plate |
Present |
Regionally restricted |
2K
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Expression is in the rostral and caudal tectum.
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| TS19: hindbrain |
Present |
Regionally restricted |
2K
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Absent at the isthmus. Expression in an area corresponding to rhombomre 1.
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| TS19: eye |
Absent |
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2K
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Specimen
3A:
embryonic day 10.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
3A
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Expression is in the lateral margin of the dorsal half of the spinal cord.
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Specimen
3B:
embryonic day 11.5
(more )
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube lateral wall |
Present |
Regionally restricted |
3B
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Expression in the lateral margin more dorsal and medial towards the dorsal roof plate.
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Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord lateral wall |
Present |
Regionally restricted |
3C
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Expression in the lateral margin more dorsal and medial towards the dorsal roof plate.
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Specimen
3D:
embryonic day 13.5
(more )
Specimen
3D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Specimen
4C:
embryonic day 11.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube |
Present |
Regionally restricted |
4C
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Expression is in two parallel stripes along the entire neural tube.
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Specimen
4C.1:
embryonic day 11.5
(more )
Specimen
4C.1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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