Fgf8 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:83255 Chi CL, et al., The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development. 2003 Jun;130(12):2633-44
Assay type:	RNA in situ
MGI Accession ID:	MGI:2677306
Gene symbol:	Fgf8
Gene name:	fibroblast growth factor 8

Probe:	Fgf8 cDNA13
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

5I ©

Specimen 5I: embryonic day 8.0 (more

)
Note: 7 somites. Littermate control; exact genotype not specified.

Structure	Level	Pattern	Image	Note
TS12: future brain	Present		5I

5J ©

Specimen 5J: embryonic day 8.0; Fgf8^tm1.3Mrt/Fgf8^tm1.4Mrt, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant. 7 somites.

Structure	Level	Pattern	Image	Note
TS12: future brain	Present		5J	Expression levels are at the same levels as seen in controls.

5Q ©

Specimen 5Q: embryonic day 9.0 (more

)
Note: 16 somites. Littermate control; exact genotype not specified.

Structure	Level	Pattern	Image	Note
TS14: future brain	Present		5Q

5R ©

Specimen 5R: embryonic day 9.0; Fgf8^tm1.3Mrt/Fgf8^tm1.4Mrt, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant. 16 somites.

Structure	Level	Pattern	Image	Note
TS14: future brain	Weak	Regionally restricted	5R	In the mutant, expression is present in only a small patch.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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