Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79394
Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676616
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Gene symbol:
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Bmp4
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Gene name:
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bone morphogenetic protein 4
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Probe:
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Bmp4 probe19
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5C:
embryonic day 9.0
(more  )
Note: 15 somite stage.
Specimen
5C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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15 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Present |
Regionally restricted |
5C
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Expressed at low levels at dorsal midline of telencephalon and anterior diencephalon (P3 and anterior P2); but not in caudal diencephalon (posterior P2 and P1). No expression in dorsal midline of diencephalon.
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| TS14: prosencephalon |
Present |
Regionally restricted |
5C
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Expressed at low levels at dorsal midline of telencephalon and anterior diencephalon (P3 and anterior P2); but not in caudal diencephalon (posterior P2 and P1). No expression in dorsal midline of diencephalon.
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| TS14: future midbrain |
Absent |
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5C
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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15 somite stage.
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Specimen
5E:
embryonic day 9.0
(more )
Note: 19 somite stage.
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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19 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Present |
Regionally restricted |
5E
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Expressed at low levels at dorsal midline of telencephalon and anterior diencephalon (P3 and anterior P2); but not in caudal diencephalon (posterior P2 and P1). No expression in dorsal midline of diencephalon.
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| TS15: future forebrain |
Present |
Regionally restricted |
5E
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Expressed at low levels at dorsal midline of telencephalon and anterior diencephalon (P3 and anterior P2); but not in caudal diencephalon (posterior P2 and P1). No expression in dorsal midline of diencephalon.
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| TS15: future midbrain |
Absent |
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5E
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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19 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Present |
Regionally restricted |
5F
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Significant upregulation of expression in diencephalic region and midbrain.
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| TS14: future midbrain |
Present |
Regionally restricted |
5F
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Significant upregulation of expression in diencephalic region and midbrain.
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Analysis Tools