Probe:	Fam probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Uniform	5_E9.5

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Strong		5_E10.5
TS17: central nervous system	Strong		5_E10.5
TS17: limb	Strong		5_E10.5
TS17: body wall mesenchyme	Absent		5_E10.5

Structure	Level	Pattern	Image	Note
TS19: neural tube	Weak	Regionally restricted	5_E11.5	Expression is increasingly caudally restricted.
TS19: tail neural tube	Weak	Regionally restricted	5_E11.5	Expression is increasingly caudally restricted.
TS19: midbrain	Present		5_E11.5
TS19: telencephalon	Present		5_E11.5
TS19: eye	Strong		5_E11.5
TS19: branchial arch	Strong	Single cells	5_E11.5	Expression is lost in the proximal half.
TS19: limb	Strong	Single cells	5_E11.5	Expression is lost in the proximal half.

Structure	Level	Pattern	Image	Note
TS20: eye	Moderate		5_E12.5
TS20: midbrain	Moderate		5_E12.5
TS20: forelimb interdigital region	Moderate	Regionally restricted	5_E12.5	Expression is in the apoptotic region.
TS20: hindlimb interdigital region	Moderate	Regionally restricted	5_E12.5	Expression is in the apoptotic region.
TS20: telencephalon	Present		5_E12.5

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	6B	Expression is in cells lying increasingly proximal to the midline.
TS20: metencephalon	Present	Regionally restricted	6B	Expression is in cells lying increasingly proximal to the midline.

Structure	Level	Pattern	Image	Note
TS20: midbrain ventricular layer	Strong	Single cells	6C
TS20: midbrain mantle layer	Moderate	Single cells	6C

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present	Regionally restricted	7B	There is no expression in the central area.
TS20: neural retinal epithelium	Strong		7B

Structure	Level	Pattern	Image	Note
TS22: pigmented retinal epithelium	Absent		7C
TS22: neural retinal epithelium	Present	Regionally restricted	7C	Expression is in the outer nuclear layer that will give rise to the photoreceptors.

Structure	Level	Pattern	Image	Note
TS22: liver	Strong	Uniform	8B

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Strong		8D
TS22: lung mesenchyme	Moderate		8D

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Strong	Regionally restricted	8E	Expression is strongest in the cells of the outer root sheath.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Uniform
TS11: extraembryonic component	Weak	Uniform
TS11: heart	Absent

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS21: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Strong	Uniform
TS17: tail central nervous system	Strong	Uniform
TS17: heart	Absent

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted		Expression is restricted to cells lying increasingly proximal to the spinal canal.
TS19: tail neural tube	Present	Uniform
TS19: lens vesicle	Present	Uniform
TS19: neural retinal epithelium	Present	Uniform
TS19: heart	Absent