Probe:	Gdf11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo	Weak	Uniform

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural fold	Present	Regionally restricted	3A	Expression is in the dorsal tailbud.
TS13: embryo mesenchyme	Present	Regionally restricted	3A	Expression is in the tail bud.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural fold	Present	Regionally restricted	3B	Expression is dorsal.
TS14: tail bud	Present	Regionally restricted	3B	Expression is in mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present	Regionally restricted	3C	Expression is dorsal.
TS15: tail neural tube	Present	Regionally restricted	3C	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS17: tail mesenchyme	Present	Regionally restricted	3D	Expression is dorsal.
TS17: tail neural tube	Present	Regionally restricted	3D	Expression is dorsal.
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	3D
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	3D
TS17: 2nd branchial arch	Present	Regionally restricted	3D

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme	Present	Regionally restricted	3E	Expression is posterior and dorsal.
TS19: tail neural tube	Present	Regionally restricted	3E	Expression is posterior and dorsal.

Structure	Level	Pattern	Image	Note
TS20: tail mesenchyme	Present	Regionally restricted	3F	Expression is posterior and dorsal.
TS20: tail nervous system	Present	Regionally restricted	3F	Expression is posterior and dorsal.

Structure	Level	Pattern	Image	Note
TS14: tail bud	Present	Regionally restricted	3G	Expression is in the dorsal neural fold and the dorsal mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present		3H
TS15: tail	Present	Regionally restricted	3H	Expression is in the ectoderm.

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present		3I
TS15: tail neural tube	Present		3I

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present	Regionally restricted	3J	Expression is in the tail bud.
TS13: future spinal cord neural fold	Present	Regionally restricted	3J	Expression is in the tail bud.

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present	Regionally restricted	3K	Expression is dorsal.
TS15: tail neural tube	Present	Regionally restricted	3K	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS17: tail mesenchyme	Present	Regionally restricted	3L	Expression is dorsal.
TS17: tail neural tube	Present	Regionally restricted	3L	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS19: tail mesenchyme	Present	Regionally restricted	3M	Expression is dorsal.
TS19: tail neural tube	Present	Regionally restricted	3M	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Present	Graded	4A	The dorsal aspect is stronger than the ventral.
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4A	Expression is adjacent to the ectoderm. Expression in the dorsal aspect is stronger than the ventral.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	4B	Higher expression is seen in the posterior.

Structure	Level	Pattern	Image	Note
TS20: hand	Present	Regionally restricted	4C	Expression is in ectoderm and underlying mesenchyme.
TS20: forelimb interdigital region mesenchyme	Present		4C

Structure	Level	Pattern	Image	Note
TS21: hand	Present	Regionally restricted	4D	Expression is in the ectoderm and underlying mesenchyme.
TS21: forelimb interdigital region mesenchyme	Present		4D