Nkx2-6 RNA in situ Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:45685 Nikolova M, et al., Nkx2.6 expression is transiently and specifically restricted to the branchial region of pharyngeal-stage mouse embryos. Mech Dev. 1997 Dec;69(1-2):215-8
Assay type:	RNA in situ
MGI Accession ID:	MGI:2429957
Gene symbol:	Nkx2-6
Gene name:	NK2 homeobox 6

Probe:	Nkx2-6 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1A ©

Specimen 1A: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS12: branchial arch	Present	Regionally restricted	1A	Expression is restricted to the branchial region directly underlying the heart.

1B ©

Specimen 1B: embryonic day 8.75 (more

)

Structure	Level	Pattern	Image	Note
TS12: branchial arch	Present	Regionally restricted	1B	Expression is restricted to the branchial region directly underlying the heart.

1C ©

Specimen 1C: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Absent		1C
TS15: 1st branchial arch maxillary component	Absent		1C
TS15: 2nd branchial arch	Present		1C
TS15: 3rd branchial arch	Present		1C

1D ©

Specimen 1D: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS16: 1st branchial arch mandibular component	Absent		1D
TS16: 1st branchial arch maxillary component	Absent		1D
TS16: 2nd branchial arch	Present		1D
TS16: 3rd branchial arch	Present		1D

1E ©

Specimen 1E: embryonic day 10.0 (more

)

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial arch	Present		1E
TS17: 3rd branchial arch	Present		1E
TS17: 4th branchial arch	Present		1E

1F ©

Specimen 1F: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial arch	Trace		1F
TS17: 3rd branchial arch	Trace		1F
TS17: 4th branchial arch	Trace		1F

2A ©

Specimen 2A: embryonic day 8.5 (more

)
Note: The embryos were embedded in araldite.

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Present	Regionally restricted	2A	Expression is restricted to the branchial region directly underlying the heart.

2B ©

Specimen 2B: embryonic day 9.0 (more

)
Note: The embryos were embedded in araldite.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Absent		2B
TS15: 1st branchial arch maxillary component	Absent		2B
TS15: 2nd branchial arch	Present		2B
TS15: 3rd branchial arch	Present		2B

2C ©

Specimen 2C: embryonic day 9.0 (more

)
Note: The embryos were embedded in araldite.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Absent		2C
TS15: 1st branchial arch maxillary component	Absent		2C
TS15: 2nd branchial arch	Present		2C
TS15: 3rd branchial arch	Present		2C

2D ©

Specimen 2D: embryonic day 9.5 (more

)
Note: The embryos were embedded in araldite.

Structure	Level	Pattern	Image	Note
TS15: 2nd branchial arch	Present		2D
TS15: 3rd branchial arch	Present		2D

2E ©

Specimen 2E: embryonic day 10.0 (more

)
Note: The embryos were embedded in araldite.

Structure	Level	Pattern	Image	Note
TS16: 2nd branchial arch ectoderm	Strong		2E
TS16: 3rd branchial arch ectoderm	Strong		2E
TS16: 4th branchial arch ectoderm	Strong		2E
TS16: 2nd branchial arch mesenchyme	Present		2E
TS16: 3rd branchial arch mesenchyme	Present		2E
TS16: 4th branchial arch mesenchyme	Present		2E
TS16: 2nd branchial arch endoderm	Trace		2E
TS16: 3rd branchial arch endoderm	Trace		2E
TS16: 4th branchial arch endoderm	Trace		2E

2F ©

Specimen 2F: embryonic day 10.0 (more

)
Note: The embryos were embedded in araldite.

Structure	Level	Pattern	Image	Note
TS16: 2nd branchial arch ectoderm	Strong		2F
TS16: 3rd branchial arch ectoderm	Strong		2F
TS16: 4th branchial arch ectoderm	Strong		2F
TS16: 2nd branchial arch mesenchyme	Present		2F
TS16: 3rd branchial arch mesenchyme	Present		2F
TS16: 4th branchial arch mesenchyme	Present		2F
TS16: 2nd branchial arch endoderm	Trace		2F
TS16: 3rd branchial arch endoderm	Trace		2F
TS16: 4th branchial arch endoderm	Trace		2F

Specimen Not shown: embryonic day 7.5 (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Specimen Not shown: embryonic day 8.0 (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent

Specimen Not shown: embryonic day 11.0 (more

)

Structure	Level	Pattern	Image	Note
TS18: embryo	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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